Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/35877
Title: Impact of neonatal malnutrition on expression TLR-9, NF-kB and cytokines of macrophages infected in vitro with methicillin resistant Staphylococcus aureus
Authors: Morais, Natália Gomes de
Costa, Thacianna Barreto da
Lima, Luiz Felipe Ferreira de
Basílio, Dyowani dos Santos
Morais, Nadja Nara Gomes de
Cavalcanti, Milena de Paiva
Pereira, Valéria Rêgo Alves
Castro, Celia Maria Machado Barbosa de
Affilliation: Universidade Federal da Bahia. Salvador, BA, Brasil.
Universidade Federal de Pernambuco. Recife, PE, Brasil.
Universidade Federal do Vale São Francisco. Petrolina, PE, Brasil.
Universidade Federal do Vale São Francisco. Petrolina, PE, Brasil.
Universidade Federal Rural de Pernambuco. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisa Aggeu Magalhães. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisa Aggeu Magalhães. Recife, PE, Brasil.
Universidade Federal de Pernambuco. Recife, PE, Brasil.
Abstract: Early nutritional aggressions promote epigenetic adjustments that culminate in the loss of phenotype plasticity (with permanent long-term modifications). Maternal diet and inadequate neonatal nutrition can result in fetal programming that presents susceptibility to infections in adult life. Thus, it becomes essential to verify the impacts of neonatal malnutrition (even following nutritional replacement) on the immunological response to methicillin resistant Staphylococcus aureus (MRSA) infections. Male rats were divided into two distinct groups: Nourished and Malnourished. After isolation of mononuclear cells, four systems were established: negative control, positive control and two testing systems, (MSSA and MRSA). Tests were performed to analyze expression of TLR-9, NF-kB, IL-1β, IL-18 and IL-33. For statistical analysis, we used the Student t and ANOVA tests p < 0.05. Even after nutritional replacement, malnutrition in the neonatal period compromised the animals' weight gains p < 0.05. There was a reduction in the expression of the immunological response in the positive control, however deregulation was observed in the gene expression of MRSA-infected macrophages, with a reduction in TLR-9 expression, and overexpression in NF-kB and cytokines p < 0.05. Puppies inflicted with protein-calorie malnutrition were compromised; (long-term) body growth and immune response. In the infectious scenario, immune collapse is reflected in inflammatory response exacerbation with a likely histolytic character. Immune disabling (resulting from gene expression deregulation) causes susceptibility to infections due to ineffective recognition, intense pro-inflammatory mediation, and cell death. It is suggested that neonatal malnutrition can program susceptibility to multiresistant bacterial infections, and generally favors a triggering of more intense confrontations with fatal outcomes.
Keywords: MRSA
Macrophage
Malnutrition
Plasticity
DeCS: Animais
Animais Recém-Nascidos
Peso Corporal
Citocinas
Metabolismo
Cães
Regulação da Expressão Gênica
Interleucina-18
Interleucina-1beta
Interleucina-33
Macrófagos
Microbiologia
Macrófagos Alveolares
Masculino
Desnutrição
Staphylococcus aureus Resistente à Meticilina
Patogenicidade
NF-kappa B
Ratos
Ratos Wistar
Infecções Estafilocócicas
Imunologia
Receptor Toll-Like 9
Issue Date: 2019
Citation: MORAIS, Natália Gomes de et al. Impact of neonatal malnutrition on expression TLR-9, NF-kB and cytokines of macrophages infected in vitro with methicillin resistant Staphylococcus aureus. Microbial Pathogenesis, v. 132, p. 254–260, July 2019.
DOI: 10.1016/j.micpath.2019.05.009
ISSN: 0882-4010
Copyright: restricted access
Appears in Collections:PE - IAM - Artigos de Periódicos




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