Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/36335
Title: Evidence of Different IL-1 beta Activation Pathways in Innate Immune Cells From Indeterminate and Cardiac Patients With Chronic Chagas Disease
Authors: Medeiros, Nayara Ingrid
Pinto, Bruna Fernandes
Santos, Silvana Maria Elói
Carvalho, Andréa Teixeira de
Magalhães, Luísa M. D.
Dutra, Walderez Ornelas
Oliveira, Rodrigo Corrêa de
Gomes, Juliana Assis S.
Affilliation: Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, Brasil.
Universidade Federal de Minas Gerais. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de Propedêutica Complementar. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil.
Inflammatory Cell Dynamics Section. Center for Cancer Research. National Institute of Health. NIH, Bethesda, MD, USA.
Universidade Federal de Minas Gerais. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, Brasil / Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil / Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Belo Horizonte, MG, Brasil.
Universidade Federal de Minas Gerais. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, Brasil.
Abstract: Background: Chagas cardiomyopathy is the main fibrosing myocarditis among known heart diseases. Development of cardiomyopathy has been related to extracellular matrix (ECM) remodeling, which are controlled by matrix metalloproteinases (MMPs) and cytokines, especially interleukin (IL)-1β. The convertion of 31KDa inactive precursor, the proIL-1β in 17KDa active IL-1β peptide, is controlled by caspase-1-dependent pathway, associated with inflammasomes. Other caspase-1 independent mechanisms mediated by proteases, especially as MMPs, have already been described. Methods: We evaluated IL-1β activation pathways in neutrophils and monocyte subsets from patients with different clinical forms of Chagas disease after T. cruzi antigen stimulation by multiparameter flow cytometry. Results: Our data demonstrated that Chagas patients with the indeterminate clinical form (IND) showed increased levels of IL-1β post-stimulation as well as increased expression of MMP-2, NLRP3, and CASP1, which are associated with the classical caspase-1-dependent pathway. Conversely, patients with the cardiac clinical form (CARD) showed increased IL-1β after stimulation associated with MMP-9 and alternative caspase-1-independent pathway. Conclusions: We suggest some distinct molecular mechanisms for production of IL-1β in innate immune cells from patients with different clinical forms of Chagas disease. MMP-2 and MMP-9 gelatinases are associated with distinct disease outcomes and IL-1β production.
Keywords: Chagas disease
IL-1β activation pathways
Caspase-1-independent
Chagasic cardiomyopathy
Chagasic cardiomyopathy
Gelatinases
Issue Date: 2019
Publisher: Frontiers Research Foundation
Citation: MEDEIROS, Nayara Ingrid et al. Evidence of Different IL-1 beta Activation Pathways in Innate Immune Cells From Indeterminate and Cardiac Patients With Chronic Chagas Disease. Frontiers in Immunology, v. 10, p. 1-11, 2019.
DOI: 10.3389/fimmu.2019.00800
ISSN: 1664-3224
Copyright: open access
Appears in Collections:MG - IRR - Artigos de Periódicos

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