Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/36354
Title: Transcriptomic profile of two developmental stages of the cestode parasite Mesocestoides corti
Authors: Cabrera, Tatiana Noel Basika
Paludo, G. P.
Araujo, Flavio Marcos
Salim, Anna Christina de Matos
Pais, Fabiano Sviatopolk Mirsky
Maldonado, L.
Macchiaroli, N.
Lima, J. Camargo de
Rosenzvit, M.
Oliveira, Guilherme Corrêa de
Kamenetzky, L.
Ferreira, Henrique Bunselmeyer
Affilliation: Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Laboratório de Genômica Estrutural e Funcional. Porto Alegre, RS, Brasil / Universidade Federal do Rio Grande do Sul. Laboratório de Biologia Molecular de Cestódeos. Porto Alegre, RS, Brasil / Universidade Federal do Rio Grande do Sul. Programa de Pós-Graduação em Biologia Celular e Molecular. Porto Alegre, RS, Brasil.
Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Laboratório de Genômica Estrutural e Funcional. Porto Alegre, RS, Brasil / Universidade Federal do Rio Grande do Sul. Programa de Pós-Graduação em Biologia Celular e Molecular. Porto Alegre, RS, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil
Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica. Buenos Aires, Argentina / Consejo Nacional de Investigaciones Científicas y Tecnológicas. Buenos Aires, Argentina.
Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica. Buenos Aires, Argentina/ Consejo Nacional de Investigaciones Científicas y Tecnológicas. Buenos Aires, Argentina
Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Laboratório de Genômica Estrutural e Funcional. Porto Alegre, RS, Brasil / Universidade Federal do Rio Grande do Sul. Programa de Pós-Graduação em Biologia Celular e Molecular. Porto Alegre, RS, Brasil.
Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica. Buenos Aires, Argentina / Consejo Nacional de Investigaciones Científicas y Tecnológicas. Buenos Aires, Argentina.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil / Instituto Tecnológico Vale. Belém, PA, Brasil.
Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica. Buenos Aires, Argentina / Consejo Nacional de Investigaciones Científicas y Tecnológicas. Buenos Aires, Argentina.
Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Laboratório de Genômica Estrutural e Funcional. Porto Alegre, RS, Brasil / Universidade Federal do Rio Grande do Sul. Laboratório de Biologia Molecular de Cestódeos. Porto Alegre, RS, Brasil / Universidade Federal do Rio Grande do Sul. Programa de Pós-Graduação em Biologia Celular e Molecular. Porto Alegre, RS, Brasil / Universidade Federal do Rio Grande do Sul. Instituto de Biociências. Departamento de Biologia Molecular e Biotecnologia. Porto Alegre, RS, Brasil.
Abstract: Cestode development involves complex morphological and physiological changes. Here, we performed a differential expression analysis of gene transcripts between two developmental stages of the model cestode Mesocestoides corti. A RNA-seq-based approach was used to compare the transcriptomes of the tetrathyridium (larval, TT) and strobilated worm (ST) stages of the parasite. We found 19,053 transcripts, from which ∼45% were complete matches to genes previously annotated in the available M. corti draft genome sequence, ∼24% were considered novel isoforms, and ∼24% were considered potential novel transcripts. Stage-specific transcripts were found for both TTs (66) and STs (136), along with shared transcripts significantly overrepresented in one stage (342 in TTs, and 559 in STs). Differential expression and Gene Ontology term enrichment analyzes provided evidence of upregulation of different sets of transcripts associated with ‘cytoskeleton’, ‘metabolism’ and ‘oxidation-reduction’ processes in each stage, suggesting functional involvement of the corresponding genes with stage-specific features. Transcripts and processes enriched in the TT reflect typical larval processes that occur with the parasite in the intermediate host, such as asexual reproduction and budding, as well as active migration from the peritoneum to the liver and vice versa. In STs, transcripts associated with ‘development’, ‘cell growth’, and ‘morphogenesis’ were enriched, along with processes related to sexual reproduction, represented by the upregulation of numerous transcription factors, protein kinases, and histones. Overall, our results contributed to significantly increase the knowledge on the M. corti gene repertoire and expression profile in two developmental stages. Functional implications for the biology of larval and adult cestode parasites and for host-parasite interactions are discussed.
Keywords: RNA-seq
DEG
Trascriptome
Strobilation
Segmentation
Cestoda
Issue Date: 2019
Publisher: Elsevier/North-Holland Biomedical Press
Citation: CABRERA, Tatiana Noel Basika et al. Transcriptomic profile of two developmental stages of the cestode parasite Mesocestoides corti. Molecular and Biochemical Parasitology, v. 229, p. 35-46, 2019.
DOI: 10.1016/j.molbiopara.2019.02.006
ISSN: 0166-6851
Copyright: restricted access
Appears in Collections:MG - IRR - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
Transcriptomic profile.pdf5.97 MBAdobe PDF    Request a copy



FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.