Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/36423
Title: Epidemiological profile of Zika, Dengue and Chikungunya virus infections identified by medical and molecular evaluations in Rondonia, Brazil
Authors: Vieira, Deusilene Souza
Zambenedetti, Miriam Ribas
Requião, Luciana
Borghetti, Ivo Alberto
Luna, Luciano Kleber de Souza
Santos, Alcione de Oliveira dos
Taborda, Roger Lafontaine Mesquita
Pereira, Dhelio Batista
Krieger, Marco Aurélio
Salcedo, Juan Miguel Villalobos
Rampazzo, Rita de Cássia Pontello
Affilliation: Fundação Oswaldo Cruz. Escritório Rondônia. Porto Velho, RO, Brasil / Universidade Federal de Rondônia. Programa de Pós-Graduação em Biologia Experimental. Porto Velho, RO, Brasil / Centro de Pesquisa em Medicina Tropical. Porto Velho, RO, Brasil.
Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.
Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.
Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil / Universidade Federal do Paraná. Departamento de Engenharia de Bioprocessos e Biotecnologia. Curitiba, PR, Brasil.
Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Escritório Rondônia. Porto Velho, RO, Brasil / Centro de Pesquisa em Medicina Tropical. Porto Velho, RO, Brasil.
Centro de Pesquisa em Medicina Tropical. Porto Velho, RO, Brasil.
Centro de Pesquisa em Medicina Tropical. Porto Velho, RO, Brasil.
Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Escritório Rondônia. Porto Velho, RO, Brasil / Centro de Pesquisa em Medicina Tropical. Porto Velho, RO, Brasil.
Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.
Abstract: Several arboviruses have emerged and/or re-emerged in North, Central and South-American countries. Viruses from some regions of Africa and Asia, such as the Zika and Chikungunya virus have been introduced in new continents causing major public health problems. The aim of this study was to investigate the presence of RNA from Zika, Dengue and Chikungunya viruses in symptomatic patients from Rondonia, where the epidemiological profile is still little known, by one-step real-time RT-PCR. The main clinical signs and symtoms were fever (51.2%), headache (78%), chills (6.1%), pruritus (12.2%), exanthema (20.1%), arthralgia (35.3%), myalgia (26.8%) and retro-orbital pain (19.5%). Serum from 164 symptomatic patients were collected and tested for RNA of Zika, Dengue types 1 to 4 and Chikungunya viruses, in addition to antibodies against Dengue NS1 antigen. Direct microscopy for Malaria was also performed. Only ZIKV RNA was detected in 4.3% of the patients, and in the remaining 95.7% of the patients RNA for Zika, Dengue and Chikungunya viruses were not detected. This finding is intriguing as the region has been endemic for Dengue for a long time and more recently for Chikungunya virus as well. The results indicated that medical and molecular parameters obtained were suitable to describe the first report of symptomatic Zika infections in this region. Furthermore, the low rate of detection, compared to clinical signs and symptoms as the solely diagnosis criteria, suggests that molecular assays for detection of viruses or other pathogens that cause similar symptoms should be used and the corresponding diseases could be included in the compulsory notification list.
Keywords: Zika Virus
Chikungunya virus
Real-Time Polymerase Chain Reaction
Arbovirus Infections
Pathology, Molecular
Keywords in spanish: Virus Zika
Virus Chikungunya
Reacción en Cadena en Tiempo Real de la Polimerasa
Infecciones por Arbovirus
Patología Molecular
keywords: Dengue
DeCS: Zika virus
Vírus Chikungunya
Reação em Cadeia da Polimerase em Tempo Real
Infecções por Arbovirus
Patologia Molecular
Issue Date: 2019
Publisher: Universidade de São Paulo
Citation: VIEIRA, Deusilene Souza et al. Epidemiological profile of Zika, Dengue and Chikungunya virus infections identified by medical and molecular evaluations in Rondonia, Brazil. Revista do Instituto de Medicina Tropical de São Paulo, São Paulo. v. 61, n. 40, p. 1-62, 2019.
DOI: 10.1590/S1678-9946201961040
ISSN: 0036-4665
Copyright: open access
Appears in Collections:RO - Artigos de Periódicos
PR - ICC - Artigos de Periódicos

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