Rapid Identification of Sporothrix species by T3B fingerprinting

Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
Universidade do Minho. Molecular and Environmental Biology Centre. Braga, Portugal.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
Universidade do Minho. Molecular and Environmental Biology Centre. Braga, Portugal.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.

Abstract

This article describes PCR fingerprinting using the universal primer T3B to distinguish among species of the Sporothrix complex, S. brasiliensis, S. globosa, S. mexicana, and S. schenckii. This methodology generated distinct banding patterns, allowing the correct identification of all 35 clinical isolates at the species level, confirmed by partial calmodulin (CAL) gene sequence analyses. This methodology is simple, reliable, rapid, and cheap, making it an ideal routine identification system for clinical mycology laboratories.

Keywords

Sporothrix species
Sporotrichosis

Publisher

American Society for Microbiology

Citation

OLIVEIRA, Manoel Marques Evangelista de et al. Rapid identification of Sporothrix species by T3B fingerprinting. Journal of Clinical Microbiology, v. 50, n. 6, p. 2159-2162, June 2012.

DOI

10.1128/JCM.00450-12

ISSN

0095-1137

Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/36571

Type

Copyright

Open access

Embargo date

2012-04-18

Collections

Share

Statistics

Metadata

Author

Affilliation