| Author | Borges, Ana Maria Tavares | |
| Author | Teixeira, José Marcus Sócrates | |
| Author | Costa, Paulo Sérgio Sucasas da | |
| Author | Giugliano, Loreny Gimenes | |
| Author | Fiaccadori, Fabíola Souza | |
| Author | Franco, Rachel de Carvalho e | |
| Author | Brito, Wilia Marta Elsner Diederichsen de | |
| Author | Leite, José Paulo Gagliardi | |
| Author | Cardoso, Divina das Dores de Paula | |
| Access date | 2019-10-22T12:15:00Z | |
| Available date | 2019-10-22T12:15:00Z | |
| Document date | 2006 | |
| Citation | BORGES, Ana Maria Tavares et al. Detection of calicivirus from fecal samples from children with acute gastroenteritis in the West Central region of Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 101, n. 7, p. 721-724, Nov. 2006. | pt_BR |
| ISSN | 0074-0276 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/36619 | |
| Language | eng | pt_BR |
| Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Calicivirus | pt_BR |
| Subject in Portuguese | Gastroenterite aguda | pt_BR |
| Subject in Portuguese | Reação em cadeia da transcriptase-polimerase reversa | pt_BR |
| Subject in Portuguese | Brasil | pt_BR |
| Title | Detection of calicivirus from fecal samples from children with acute gastroenteritis in the West Central region of Brazil | pt_BR |
| Type | Article | |
| DOI | 10.1590/S0074-02762006000700003 | |
| Abstract | The objective of this study was to describe the circulation of caliciviruses in the West Central region of Brazil and
its correlation with children s gender and age, as well as with the year and months of the sample collection. Reverse
transcriptase-polymerase chain reaction was performed to detect the human calicivirus genome in 1006 fecal
samples that were collected in Goiânia (n = 696) and Brasília (n = 310). Viral RNA was detected in 8.6% of the
samples. No significant difference in viral prevalence was found regarding gender, age or year of the sample.
However, it was observed that in Goiânia, there is a higher incidence of caliciviruses from September to March. The
analysis employing three primer pairs demonstrated that the Ni/E3 or JV12/13 primer pairs, which detect norovirus
(NoV), detected 41 positive samples while the 289/290 primer pair, which detects NoV or sapovirus, detected the
remaining 46 samples. Calicivirus circulates in the West Central region of Brazil and for better detection of this
virus it is important to use more than one primer pair. Also, we conclude that the seasonality presented by this virus
is related to higher humidity in the period. | pt_BR |
| Affilliation | Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Laboratório de Virologia. Goiânia, GO, Brasil. | pt_BR |
| Affilliation | Instituto de Saúde do Distrito Federal. Brasília, DF, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Goiás. Faculdade de Medicina. Goiânia, GO, Brasil. | pt_BR |
| Affilliation | Universidade de Brasília. Departamento de Biologia Celular. Brasília, DF, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Laboratório de Virologia. Goiânia, GO, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Laboratório de Virologia. Goiânia, GO, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Laboratório de Virologia. Goiânia, GO, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Laboratório de Virologia. Goiânia, GO, Brasil. | pt_BR |
| Subject | Calicivirus | pt_BR |
| Subject | Acute gastroenteritis | pt_BR |
| Subject | Reverse transcriptase-polymerase chain reaction | pt_BR |
| Subject | Brazil | pt_BR |
| e-ISSN | 1678-8060 | |
