Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/36621
Title: Detection and molecular characterization of group A rotavirus from hospitalized children in Rio de Janeiro, Brazil, 2004
Authors: Costa, Filipe Anibal Carvalho
Assis, Rosane Maria
Fialho, Alexandre Madi
Bóia, Márcio Neves
Alves, Daniele Pires Dias
Martins, Carolina Maria Miranda de Assis
Leite, José Paulo Gagliardi
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Laboratório de Virologia Comparada. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Laboratório de Virologia Comparada. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Laboratório de Virologia Comparada. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Laboratório de Doenças Parasitárias. Rio de Janeiro, RJ, Brasil.
Hospital Municipal Souza Aguiar. Rio de Janeiro, RJ, Brasil.
Hospital Municipal Salles Netto. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Laboratório de Virologia Comparada. Rio de Janeiro, RJ, Brasil.
Abstract: Rotavirus is a major cause of infantile acute diarrhea, causing about 440,000 deaths per year, mainly in developing countries. The World Health Organization has been recommending the assessment of rotavirus burden and strain characterization as part of the strategies of immunization programs against this pathogen. In this context, a prospective study was made on a sample of 134 children with acute diarrhea and severe dehydration admitted to venous fluid therapy in two state hospitals in Rio de Janeiro, Brazil, from February to September 2004. Rotavirus where detected by polyacrylamide gel electrophoresis (PAGE) and by an enzyme-linked immunoassay to rotavirus and adenovirus (EIARA) in 48% of the children. Positive samples for group A rotavirus (n = 65) were analyzed by reverse transcription/heminested multiplex polymerase chain reaction to determine the frequency of G and [P] genotypes and, from these, 64 samples could be typed. The most frequent G genotype was G1 (58%) followed by G9 (40%). One mixed infection (G1/G9) was detected. The only [P] genotype identified was P[8]. In order to estimate the rotavirus infection frequency in children who acquired diarrhea as hospital infection in those hospitals, we studied 24 patients, detecting the pathogen in 41% of them. This data suggest that genotype G9 is an important genotype in Rio de Janeiro, with implications to the future strategies of vaccination against rotavirus, reinforcing the need of continuous monitoring of circulating strains of the pathogen, in a surveillance context.
Keywords: Gastroenteritis
Hospitalization
Rotavirus A
Genotypes
Rio de Janeiro
Brazil
keywords: Gastroenterite
Hospitalização
Rotavirus A
Genótipos
Rio de Janeiro
Brasil
Issue Date: 2006
Publisher: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
Citation: COSTA, Filipe Anibal Carvalho et al. Detection and molecular characterization of group A rotavirus from hospitalized children in Rio de Janeiro, Brazil, 2004. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 101, n. 3, p. 291-294, May 2006.
DOI: 10.1590/S0074-02762006000300012
ISSN: 0074-0276
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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