Author | Holguín, Angela Victoria | |
Author | Cárdenas, Pablo | |
Author | Peñaranda, Catalina Prada- | |
Author | Leite, Laura Rabelo | |
Author | Buitrago, Camila | |
Author | Clavijo, Viviana | |
Author | Oliveira, Guilherme Corrêa de | |
Author | Leekitcharoenphon, Pimlapas | |
Author | Aarestrup, Frank Møller | |
Author | Vives, Martha J. | |
Access date | 2019-11-06T14:01:23Z | |
Available date | 2019-11-06T14:01:23Z | |
Document date | 2019 | |
Citation | HOLGUÍN, Angela Victoria et al. Host Resistance, Genomics and Population Dynamics in a Salmonella Enteritidis and Phage System. Viruses, v. 11, n. 2, p. 1-11, 2019. | pt_BR |
ISSN | 1999-4915 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/36893 | |
Language | eng | pt_BR |
Publisher | MDPI | pt_BR |
Rights | restricted access | pt_BR |
Title | Host Resistance, Genomics and Population Dynamics in a Salmonella Enteritidis and Phage System | pt_BR |
Type | Article | pt_BR |
DOI | 10.3390/v11020188 | |
Abstract | Bacteriophages represent an alternative solution to control bacterial infections. When interacting, bacteria and phage can evolve, and this relationship is described as antagonistic coevolution, a pattern that does not fit all models. In this work, the model consisted of a microcosm of Salmonella enterica serovar Enteritidis and jSan23 phage. Samples were taken for 12 days every 48 h. Bacteria and phage samples were collected; and isolated bacteria from each time point were challenged against phages from previous, contemporary, and subsequent time points. The phage plaque tests, with the genomics analyses, showed a mutational asymmetry dynamic in favor of the bacteria instead of antagonistic coevolution. This is important for future phage-therapy applications, so we decided to explore the population dynamics of Salmonella under different conditions: pressure of one phage, a combination of phages, and phages plus an antibiotic. The data from cultures with single and multiple phages, and antibiotics, were used to create a mathematical model exploring population and resistance dynamics of Salmonella under these treatments, suggesting a nonlethal, growth-inhibiting antibiotic may decrease resistance to phage-therapy cocktails. These data provide a deep insight into bacterial dynamics under different conditions and serve as additional criteria to select phages and antibiotics for phage-therapy. | pt_BR |
Affilliation | Department of Biological Sciences. Universidad de Los Andes. Bogotá, Colombia. | pt_BR |
Affilliation | Department of Biological Sciences. Universidad de Los Andes. Bogotá, Colombia. | pt_BR |
Affilliation | Department of Biological Sciences. Universidad de Los Andes. Bogotá, Colombia. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Department of Biological Sciences. Universidad de Los Andes. Bogotá, Colombia. | pt_BR |
Affilliation | Department of Biological Sciences. Universidad de Los Andes. Bogotá, Colombia. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil / Instituto Tecnológico Vale. Belém, PA, Brazil. | pt_BR |
Affilliation | Research Group for Genomic Epidemiology. National Food Institute. Technical University of Denmark. Lyngby, Denmark. | pt_BR |
Affilliation | Research Group for Genomic Epidemiology. National Food Institute. Technical University of Denmark. Lyngby, Denmark. | pt_BR |
Affilliation | Department of Biological Sciences. Universidad de Los Andes. Bogotá, Colombia. | pt_BR |
Subject | Phage-therapy | pt_BR |
Subject | Salmonella Enteritidis | pt_BR |
Subject | Bacteria-phage coevolution | pt_BR |
Subject | Antibiotics | pt_BR |
Subject | Bacterial resistance | pt_BR |
Embargo date | 2022-01-01 | |