Author | Souza, Maria Auxiliadora de | |
Author | Pereira, Mirian Claudia de Souza | |
Author | Côrte-Real, Suzana | |
Access date | 2019-11-07T15:15:06Z | |
Available date | 2019-11-07T15:15:06Z | |
Document date | 1997 | |
Citation | SOUSA, Maria Auxiliadora; PEREIRA, Mirian Claudia de Souza; CÔRTE-REAL, Suzana. Leishmania major: Parasite Interactions Suggesting Sexuality. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 92, n. 6, p. 761-766, Nov./Dec. 1997. | pt_BR |
ISSN | 0074-0276 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/36944 | |
Language | eng | pt_BR |
Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Trypanosomatidae | pt_BR |
Subject in Portuguese | Leishmania major | pt_BR |
Subject in Portuguese | Sexualidade | pt_BR |
Subject in Portuguese | Morfologia | pt_BR |
Subject in Portuguese | Imunocitoquímica | pt_BR |
Subject in Portuguese | Microscopia eletrônica de varredura | pt_BR |
Title | Leishmania major: Parasite Interactions Suggesting Sexuality | pt_BR |
Type | Article | pt_BR |
DOI | 10.1590/S0074-02761997000600006 | |
Abstract | In five experiments, Leishmania (Leishmania) major (MRHO/SU/59/P-strain) grew poorly when seeded in FYTS medium supplemented with 15% fetal calf serum, but presented several peculiar pairs of promastigotes diametrically opposed and attached at their posterior ends (5.8-13.5%). As seen in Giemsa-stained smears, a ring-like line and/or an enlargement, generally occurred at the parasite junction. A close proximity of nuclei, which sometimes were difficult to distinguish from each other, was also observed at this junction. Several of these pairs appeared to be composed of fused cells in which the nuclei could be apparently fused, as shown by fluorescence microscopy to detect ß-tubulin and DNA, and by scanning electron microscopy. Under other culture conditions these pairs were absent or occurred at very low rates (0.2-2.2%). Such pairs differ markedly from longitudinally dividing cells and resemble those described in two other Leishmania species, as well as in Herpetomonas megaseliae and Phytomonas davidi, suggesting steps of a putative sexual process. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia. Coleção de Tripanossomatídeos. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Trypanosomatidae | pt_BR |
Subject | Leishmania major | pt_BR |
Subject | Sexuality | pt_BR |
Subject | Immunocytochemistry | pt_BR |
Subject | Scanning electron microscopy | pt_BR |
Subject | Morphology | pt_BR |
e-ISSN | 1678-8060 | |