Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/36944
Title: Leishmania major: Parasite Interactions Suggesting Sexuality
Authors: Souza, Maria Auxiliadora de
Pereira, Mirian Claudia de Souza
Côrte-Real, Suzana
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia. Coleção de Tripanossomatídeos. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Celular. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Celular. Rio de Janeiro, RJ. Brasil.
Abstract: In five experiments, Leishmania (Leishmania) major (MRHO/SU/59/P-strain) grew poorly when seeded in FYTS medium supplemented with 15% fetal calf serum, but presented several peculiar pairs of promastigotes diametrically opposed and attached at their posterior ends (5.8-13.5%). As seen in Giemsa- stained smears, a ring-like line and/or an enlargement, generally occurred at the parasite junction. A close proximity of nuclei, which sometimes were difficult to distinguish from each other, was also observed at this junction. Several of these pairs appeared to be composed of fused cells in which the nuclei could be apparently fused, as shown by fluorescence microscopy to detect ß-tubulin and DNA, and by scanning electron microscopy. Under other culture conditions these pairs were absent or occurred at very low rates (0.2-2.2%). Such pairs differ markedly from longitudinally dividing cells and resemble those described in two other Leishmania species, as well as in Herpetomonas megaseliae and Phytomonas davidi, suggesting steps of a putative sexual process.
Keywords: Trypanosomatidae
Leishmania major
Sexuality
immunocytochemistry
Scanning electron microscopy
Morphology
keywords: Trypanosomatidae
Leishmania major
Sexualidade
Morfologia
Imunocitoquímica
Microscopia eletrônica de varredura
Issue Date: 1997
Publisher: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
Citation: SOUSA, Maria Auxiliadora; PEREIRA, Mirian Claudia de Souza; CÔRTE-REAL, Suzana. Leishmania major: Parasite Interactions Suggesting Sexuality. Mem. Inst. Oswaldo Cruz, Rio de Janeiro, v. 92, n. 6, p. 761-766, Nov./Dec. 1997.
DOI: 10.1590/S0074-02761997000600006
ISSN: 0074-0276
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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