Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/36950
Title: Drug Resistance and Genotypes of Strains of Mycobacterium tuberculosis Isolated from Human Immunodeficiency Virusinfected and Non-infected Tuberculosis Patients in Bauru, São Paulo, Brazil
Authors: Baptista, Ida Maria Foschiani Dias
Oelemann, Maraníbia Cardoso
Opromolla, Diltor Vladimir Araújo
Suffys, Philip Noel
Affilliation: Instituto Lauro de Souza Lima. Divisão de Pesquisa e Ensino. Equipe Técnica de Microbiologia. Bauru, SP, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular e Diagnóstico de Doenças Infecciosas. Rio de Janeiro, RJ. Brasil.
Instituto Lauro de Souza Lima. Divisão de Pesquisa e Ensino. Equipe Técnica de Microbiologia. Bauru, SP, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular e Diagnóstico de Doenças Infecciosas. Rio de Janeiro, RJ. Brasil.
Abstract: Little is known about transmission and drug resistance of tuberculosis (TB) in Bauru, State of São Paulo. The objective of this study was to evaluate risk factors for transmission of Mycobacterium tuberculosis strains in this area. Strains were collected from patients attended at ambulatory services in the region and susceptibility towards the main first line antibiotics was determined and fingerprinting performed. A total of 57 strains were submitted to susceptibility testing: 23 (42.6%) were resistant to at least one drug while 3 (13%) were resistant against both rifampicin and isoniazide. Resistant strains had been isolated from patients that had not (n = 13) or had (n = 9) previously been submitted to anti-TB treatment, demonstrating a preoccupying high level of primary resistance in the context of the study. All strains were submitted to IS6110 restriction fragment length polymorphism (IS6110- RFLP) and double repetitive element PCR (DRE-PCR). Using IS6110-RFLP, 26.3% of the strains were clustered and one cluster of 3 patients included 2 HIV-infected individuals that had been hospitalized together during 16 days; clustering of strains of patients from the hospital was however not higher than that of patients attended at health posts. According to DRE-PCR, 55.3% belonged to a cluster, confirming the larger discriminatory power of IS6110- RFLP when compared to DRE-PCR, that should therefore be used as a screening procedure only. No clinical, epidemiological or microbiological characteristics were associated with clustering so risk factors for transmission of TB could not be defined in the present study.
Keywords: Tuberculosis
Transmission
Drug resistance
Fingerprinting
São Paulo
Brazil
keywords: Tuberculose
Transmissão
Resistência a medicamentos
IMpressão digital
São Paulo
Brasil
Issue Date: 2002
Publisher: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
Citation: BAPTISTA, Ida Maria Foschiani Dias et al. Drug Resistance and Genotypes of Strains of Mycobacterium tuberculosis Isolated from Human Immunodeficiency Virusinfected and Non-infected Tuberculosis Patients in Bauru, São Paulo, Brazil. Mem. Inst. Oswaldo Cruz, Rio de Janeiro, v. 97, n. 8, p. 1147-1152, Dec. 2002.
DOI: 10.1590/S0074-02762002000800015
ISSN: 0074-0276
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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