Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/36951
Title: Comparative study of RFLP-IS6110 and MIRU-VNTR from Mycobacterium tuberculosis isolated in the state of Minas Gerais, Brazil
Authors: Augusto, Cláudio José
Carvalho, Wânia da Silva
Almeida, Isabela Neves de
Figueiredo, Lida Jouca de Assis
Dantas, Nayanne Gama Teixeira
Suffys, Philip Noel
Miranda, Silvana Spíndola de
Affilliation: Fundação Ezequiel Dias. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ. Brasil.
Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
Abstract: tDNA genotyping of Mycobacterium tuberculosis has been widely applied in the understandingof disease transmission in many countries. The purpose of this study was to genotype thestrains of M. tuberculosis isolated in patients with new tuberculosis (TB) cases in Minas Gerais,as well as to compare the similarity, discriminatory power, and agreement of the clustersbetween the IS6110 Restriction Fragment Length Polymorfism (RFLP) and 12 loci VariableNumber Tandem Repeat – Mycobacterial Interspersed Repetitive Units (MIRU-VNTR) tech-niques. It was observed that 32% (66/204) of the isolated strains in the RFLP-IS6110 and50.9% (104/204) of the isolated strains in the MIRU-VNTR presented a similarity of equal toor above 85%. The RFLP-IS6110 and MIRU-VNTR proved to contain a high discriminatorypower. The similarity index resulting from the RFLP showed no recent transmission. Goodagreement was observed between the techniques when clusters were detected; however,the best epidemiological relationship was found when using the RFLP-IS6110.
Keywords: Mycobacterium tuberculosis
RFLP
VNTR loci
Brazil
keywords: Mycobacterium tuberculosis
Minas Gerais
Issue Date: 2018
Publisher: Sociedade Brasileira de Microbiologia
Citation: AUGUSTO, Cláudio José et al. Comparative study of RFLP-IS6110 and MIRU-VNTR from Mycobacterium tuberculosis isolated in the state of Minas Gerais, Brazil. Brazilian Journal of Microbiology, v. 49, p. 641-646, 2018.
DOI: 10.1016/j.bjm.2017.04.015
ISSN: 1517-8382
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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