Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/36954
Title: Sulfated fucan from marine alga inhibits HeLa cells infection by HTLV-1 free particles: semiquantitative analysis
Authors: Romanos, Maria T. V.
Andrada-Serpa, Maria J.
Mourão, Paulo A. S.
Yoneshigue-Valentin, Yocie
Pereira, Mariana S.
Santos, Norma
Wigg, Marcia D.
Affilliation: Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Microbiologia Paulo de Góes. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Pesquisa de Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Laboratório de Tecido Conjuntivo. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Biologia. Departamento de Botânica. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Laboratório de Tecido Conjuntivo. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Microbiologia Paulo de Góes. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Microbiologia Paulo de Góes. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.
Abstract: A sulfated fucan from Laminaria abyssalis marine alga prevented the interaction of HTLV-1 particles, purified from the MT-2 cell line, with HeLa cells. The infection obtained using a concentrated virus suspension was detected only by amplification of the newly synthesized HTLV-1 proviral cDNA by the nestedpolymerase chain reaction (PCR). The sulfated polysaccharide was not toxic to the cells at a concentration of 100 µg/mL and prevented infection by the viral particles when added to the cell monolayers. The proviral cDNA was only detected when the sulfated polysaccharide was added to the cells three hours post-infection, indicating that the inhibitory activity occurred in the initial stages of virus-cell interaction. Our results demonstrate, for the first time, the ability of a sulfated fucan from marine algae to inhibit virus transmission through free virus particles.
Keywords: HTLV-1
Free particles
Laminaria abyssalis
Marine algae
Sulfated fucan
Antiviral activity
Issue Date: 2011
Publisher: Elsevier
Citation: ROMANOS, Maria T. V. et al. Sulfated fucan from marine alga inhibits HeLa cells infection by HTLV-1 free particles: semiquantitative analysis. Revista Brasileira de Farmacognosia, v. 21, n. 2, p. 229-233, Mar./Apr. 2011.
DOI: 10.1590/S0102-695X2011005000055
ISSN: 0102-695X
Copyright: restricted access
Appears in Collections:INI - Artigos de Periódicos

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