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ESTUDO SÔBRE A ESPORULAÇÃO DE UMA AMOSTRA DE BACILLUS: II - IMPORTÂNCIA DOS ÍONS MN E MG NA INDUÇÃO DO PROCESSO
Alternative title
Study on the sporogenesis of a bacillus sample. I. Importance of Mn and Mg ions in induction of the processAuthor
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Abstract in Portuguese
Foi verificado que a esporogênese de B. licheniformis, amostra 23910, sòmente ocorre quando Mn[2+] ou Mg[2+] etão presentes, em meio sintético, nas concentrações de 0,1 mg%. Ambos os íons estimulam o crescimento, e no 14º dia de incubação a cultura apresenta endósporos e esporos livres, quando a concentração dos metais é de 0,1 mg%. Aumentando-se o teor dêstes íons para 1,0 mg%, esporos livres são obtidos no 10º dia de incubação. Outros íons testados como, Ca[2+], Fe[2+], Co[2+], Cu[2+], Mo[6+], Zn[2+] e B[3+] não mostraram influir no processo. Co[2+] e Cu[2+] foram tóxicos, inibindo o crescimento. O Ca[2+] pareceu não interferir na esporogênese, mas foi capaz de inibir o cresciemnto na concentração de 3mg%. O aparecimento de endósporos e esporos livres, no meio, quando Mn[2+] encontra-se a 1,0 mg%, coincide com um teor de glicose correspontente a cêrca de 12,5% da concentração inicial do açúcar. O autor sugere, também, a possibilidade do Mn[2+] ou Mg[2+] ativar a síntese do ácido dipicolínico.
Abstract
It was verified that sporogenesis in B. licheniformis, strain 2390, occurs only when Mn[2+] or Mg[2+] is present in a synthetic medium in the rates of 0,1mg% or 1,0 mg%. Both these ions stimulate the growth of the culture and after 14 days of incubation, endopores and free-spores were found in the medium, in which metals concentrations were of 0,1 mg%. Using higher concentrations of Mn[2+] or Mg[2+] such as 1,0mg%, free-spores were obtained after 10 days incubation. Other ions tested like, Ca[2+], Fe[2+], Co[2+], Cu[2+], Mo[6+], Zn[2+] and B[3+] did not influence the process. Co[2+] and Cu[2+] were toxic, inhibiting culture's growth. The ion Ca[2+] seemed not to interfere in sporogenesis, but was able to inhibit culture's growth at a concentration of 3,0 mg%. Correlation between the rate of glucose and the time of sporogenesis of B. licheniformis was found in strain 2390, when Mn[2+] was 1,0 mg%. The critical glucose concentration was about 12,5% of the total amount added initially to the culture media. The author also suggests the possibility of dipicolinic acid synthesis being activated by Mn[2+] and Mg[2+].
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