Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/3731
Title: A new miracidia hatching device for diagnosing schistosomiasis
Authors: Jurberg, Arnon Dias
Oliveira, Áureo A de
Lenzi, Henrique Leonel
Coelho, Paulo Marcos Zach
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Instituto de Pesquisa René Rachou Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Instituto de Pesquisa René Rachou Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil / Santa Casa de Misericórdia de Belo Horizonte. Belo Horizonte, MG, Brasil
Abstract: It is still imperative to develop a parasitological technique highly sensitive for diagnosing schistosomiasis in epidemiological and individual surveys. A simple and cheap hatching device with a collecting container was manufactured and tested under experimental conditions. Twelve Kato-Katz slides were performed as golden standard for comparison. Quantitative results can be carried out by counting miracidia in a plate and parasite load can be calculated (miracidia/gram of feces). Statistically significant values were higher in the hatching test. More sensitive results, with statistical significance, were achieved using 1.5 g of feces (which corresponds to 36 Kato-Katz slides) than by using the Kato-Katz method. Advantages of this technique and its limitations are presented.
Keywords: Schistosoma mansoni
Diagnosis
Feces
DeCS: Schistosoma mansoni
Diagnóstico
Fezes
Issue Date: 2008
Citation: JURBERG, Arnon D. et al. A new miracidia hatching device for diagnosing schistosomiasis. Memórias do Instituto Oswaldo Cruz. v. 103, n.1, p. 112-114, 2008.
Description: Acknowledgements to Anna Carolina Lima, from Instituto de Pesquisa René Rachou-Fiocruz, MG, for her assistance on biostatistics, Dr. Vania Dias, from Laboratório de Patologia, Instituto Oswaldo Cruz-Fiocruz, RJ, for schematic drawings of the hatching device, Mr. João de Araújo Dutra Filho, from DIRAC-Fiocruz, RJ, for manufacturing the hatching device, Ms. Vera de Paula Ribeiro, from Laboratório de Esquistossomose, Centro de Pesquisa René Rachou-FIOCRUZ, MG, and Dr. John Kusel, from the Glasgow University, for English review.
ISSN: 0074-0276
Copyright: open access
Appears in Collections:MG - IRR - Artigos de Periódicos
IOC - Artigos de Periódicos

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