Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/3738
Title: Characterization of cerebral malaria in the outbred Swiss Webster mouse infected by Plasmodium berghei ANKA
Authors: Martins, Yuri Chaves
Smith, Mary Jane
Machado, Marcelo Pelajo
Werneck, Guilherme Loureiro
Lenzi, Henrique Leonel
Daniel-Ribeiro, Claudio Tadeu
Carvalho, Leonardo José de Moura
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Departamento de Endemias Samuel Pessoa. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.
Abstract: Plasmodium berghei ANKA (PbA) infection in susceptible inbred mouse strains is the most commonly used experimental model to study pathogenesis of cerebral malaria (CM). Indeed, many concepts on mechanisms related to this complication have arisen from works using this model. Although inbred strains present several advantages and are indicated for most studies, the use of outbred models can show unique usefulness in a number of approaches such as fine post-quantitative trait loci mapping and discovery of genes relevant to CM susceptibility or resistance, as well as pharmacological and vaccine studies. Here we describe the features of PbA infection and CM incidence, and characterize the associated multiorgan pathology in the outbred Swiss Webster mouse. This model showed a sizeable (62.7%) and reproducible incidence of CM demonstrated by clinical signs and histopathological changes in brain (microhaemorrhages, oedema and vessel plugging by mononuclear cells). Major pathological changes were also observed in lungs, liver, thymus and spleen, analogous to those observed in inbred strains. Parasitaemia levels were associated with the risk of CM development, the risk being significantly higher in mice showing higher values of parasitaemia on days 6–7 of infection. This outbred CM model is then suitable for genetic, vaccine and drug studies targeting this malaria complication.
Keywords: Cerebral Malaria
Histopathology
Murine Model
Plasmodium berghei ANKA
Swiss Webster
DeCS: Malária Cerebral
Patologia
Plasmodium berghei
Issue Date: 2009
Publisher: Wiley
Citation: MARTINS, Y. C. et al. Characterization of cerebral malaria in the outbred Swiss Webster mouse infected by Plasmodium berghei ANKA. International Journal of Experimental Pathology. v. 90, n. 2, p. 119-30. Apr. 2009.
Description: Acknowledgments To Marilene Damazio dos Santos and Luiz Carlos da Silva from the Leônidas Deane Animal House for caring mice. To Belmira Santos from the Fiocruz Animal House for providing the Swiss Webster and the CBA mice. To Rodrigo Mexes from the Service of Image Treatment and Production for treating the images.
DOI: 10.1111/j.1365-2613.2008.00622.x
ISSN: 0959-9673
Copyright: open access
Appears in Collections:ENSP - Artigos de Periódicos
IOC - Artigos de Periódicos




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