Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/37521
Title: Biodistribution of bone marrow mononuclear cells in chronic chagasic cardiomyopathy after intracoronary injection
Authors: Fonseca, Lea Mirian Barbosa
Xavier, Sergio S.
Castro, Paulo Henrique Rosado de
Lima, Ronaldo S. L.
Gutfilen, Bianca
Goldenberg, Regina C. S.
Maiolino, Angelo
Chagas, Claudia L. R.
Pedrosa, Roberto C.
Carvalho, Antonio Carlos Campos de
Affilliation: Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Department of Radiology. Rio de Janeiro, RJ, Brazil.
Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Evandro Chagas. Rio de Janeiro, RJ, Brazil / Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Department of Cardiology. Rio de Janeiro, RJ, Brazil.
Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Department of Radiology. Rio de Janeiro, RJ, Brazil.
Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Department of Cardiology. Rio de Janeiro, RJ, Brazil.
Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Department of Radiology. Rio de Janeiro, RJ, Brazil.
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Cardiologia Celular e Molecular. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Department of Hematology,. Rio de Janeiro, RJ, Brazil.
Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Department of Radiology. Rio de Janeiro, RJ, Brazil.
Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Department of Cardiology. Rio de Janeiro, RJ, Brazil.
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Cardiologia Celular e Molecular. Rio de Janeiro, RJ, Brasil.
Abstract: Background: Animal and human clinical studies have indicated that bone marrow (BM) mononuclear cell (MNC) therapy for Chagasic Cardiomyopathy (ChC) is feasible, safe and potentially efficacious. Nevertheless, little is known about the retention of these cells after intracoronary (IC) infusion. Methods: Our study investigated the homing of technetium-99m (99mTc) labeled BM MNCs and compared itto thallium-201 (201Tl) myocardial perfusion images using the standard 17-segment model. Six patients with congestive heart failure of chagasic etiology were included. Results: Scintigraphic images revealed an uptake of 5.4%±1.7, 4.3%±1.5 and 2.3%±0.6 of the total infused radioactivity in the heart after 1, 3 and 24 h, respectively. The remaining activity was distributed mainly to the liver and spleen. Of 102 segments analyzed, homing took place in 36%. Segments with perfusion had greater homing (58.6%) than those with decreased or no perfusion (6.8%), p< 0.0001. There was no correlation between the number of injected cells and the number of segments with homing for each patient (r=−0.172, p=0.774). Conclusions:These results indicate that 99mTc-BM MNCs delivered by IC injection homed to the chagasicmyocardium. However, cell biodistribution was heterogeneous and limited, being strongly associated withthe myocardial perfusion pattern at rest. These initial data suggest that the IC route may present limitationsin chagasic patients and that alternative routes of cell administration may be necessary.
Keywords: Homing
Cell therapy
Intracoronary infusion
Myocardial Scintigraphy
Chagasic cardiomyopathy
Issue Date: 2011
Publisher: Elsevier
Citation: FONSECA, Lea Mirian Barbosa et al. Biodistribution of bone marrow mononuclear cells in chronic chagasic cardiomyopathy after intracoronary injection. International Journal of Cardiology, v. 149, p. 310-314, 2011.
DOI: 10.1016/j.ijcard.2010.02.008
ISSN: 0167-5273
Copyright: restricted access
Appears in Collections:INI - Artigos de Periódicos

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