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2020-12-10
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PATHOGENESIS OF PULMONARY CRYPTOCOCCUS GATTII INFECTION: A RAT MODEL
Krockenberger, Mark B. et al. | Date Issued: 2010
Author
Krockenberger, Mark B.
Malik, Richard
Ngamskulrungroj, Popchai
Trilles, Luciana
Escandon, Patricia
Dowd, Susan
Allen, Chris
Himmelreich, Uwe
Canfield, Paul J.
Sorrell, Tania C.
Meyer, Wieland
Affilliation
University of Sydney. Faculty of Veterinary Science. Sydney, NSW, Australia.
University of Sydney. Centre for Veterinary Education. Sydney, NSW, Australia.
University of Sydney. Western Clinical School. Sydney, NSW, Australia / Westmead Millennium Institute. Centre for Infectious Diseases and Microbiology. Molecular Mycology Research Laboratory. Sydney, NSW, Australia / Mahidol University. Faculty of Medicine. Siriraj Hospital. Bangkok, Thailand.
University of Sydney. Western Clinical School. Sydney, NSW, Australia / Westmead Millennium Institute. Centre for Infectious Diseases and Microbiology. Molecular Mycology Research Laboratory. Sydney, NSW, Australia / Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
University of Sydney. Western Clinical School. Sydney, NSW, Australia / Westmead Millennium Institute. Centre for Infectious Diseases and Microbiology. Molecular Mycology Research Laboratory. Sydney, NSW, Australia / Instituto Nacional de Salud. Grupo de Microbiología. Bogotá, Colombia.
University of Sydney. Western Clinical School. Sydney, NSW, Australia / Westmead Millennium Institute. Centre for Infectious Diseases and Microbiology. Molecular Mycology Research Laboratory. Sydney, NSW, Australia.
University of Sydney. Western Clinical School. Sydney, NSW, Australia / Westmead Millennium Institute. Centre for Infectious Diseases and Microbiology. Molecular Mycology Research Laboratory. Sydney, NSW, Australia / Royal Children’s Hospital. Brisbane, QLD, Australia.
Max Planck Institute for Neurological Research. Köln, Germany.
University of Sydney. Faculty of Veterinary Science. Sydney, NSW, Australia.
University of Sydney. Western Clinical School. Sydney, NSW, Australia / Westmead Millennium Institute. Centre for Infectious Diseases and Microbiology. Sydney, NSW, Australia.
University of Sydney. Western Clinical School. Sydney, NSW, Australia / Westmead Millennium Institute. Centre for Infectious Diseases and Microbiology. Molecular Mycology Research Laboratory. Sydney, NSW, Australia.
Abstract
A model of pulmonary cryptococcosis in immunocompetent rats was developed to better understand the virulence of Cryptococcus gattii. Six isolates were studied, representing four molecular genotypes (VGI-MATa, VGIIa-MATa, VGIIa-MATa, VGIIbMATa), obtained from Australia, Vancouver (Canada) and Colombia. These originated from human patients, a cat and the environment and were administered intratracheally (i.t.) or transthoracically into Fischer 344 or Wistar-Furth rats in doses varying from 104 to 107 colony-forming units (CFU) in 0.1 ml of saline. With the exception of animals given the VGIIa-MATa isolate, rats consistently became ill or died of progressive cryptococcal pneumonia following i.t. doses exceeding 107 CFU. Affected lungs increased in weight up to tenfold and contained numerous circumscribed, gelatinous lesions. These became larger and more extensive, progressing from limited hilar and/or tracheal lesions, to virtually confluent gelatinous masses. Disease was localized to the lungs for at least 3–4 weeks, with dissemination to the brain occurring in some animals after day 29. The dose–response relationship was steep for two VGI isolates studied (human WM179, environmental WM276); doses up to 106 CFU i.t. did not produce lesions, while 107 or more yeast cells produced progressive pneumonia. Intratracheal inoculation of rats with C. gattii provides an excellent model of human pulmonary cryptococcosis in healthy hosts, mimicking natural infections. Disease produced by C. gattii in rats is distinct from that caused by C. neoformansin that infections are progressive and ultimately fatal.
Keywords
Cryptococcus
Cryptococcosis gattii
Rat
Lung
Meningitis
 
Publisher
Springer Verlag
Citation
KROCKENBERGER, Mark B. et al. Pathogenesis of Pulmonary Cryptococcus gattii infection: a rat model. Mycopathologia, v. 170, p. 315-330, 2010.
DOI
10.1007/s11046-010-9328-z
ISSN
0301-486X
Notes
Luciana Trilles. Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta a informação no documento.