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FLOW CYTOMETRY EVALUATION OF THE T-CELL RECEPTOR Vβ REPERTOIRE AMONG HIV-1 INFECTED INDIVIDUALS BEFORE AND AFTER ANTIRETROVIRAL THERAPY
Author
Affilliation
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Departamento de Micro-Imuno-Parasitologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Departamento de Doenças Infecciosas. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Departamento de Micro-Imuno-Parasitologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Departamento de Doenças Infecciosas. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.
Abstract
HIV-1 infection leads to serious impairment of the immune system
and perturbations in the T cell receptorVβ repertoire are also
described. Immune reconstitution can be potentially achieved
in response to HAART. In the present study 10 patients were
investigated for the Vβ pattern expression before and after six
months of HAART. TCR were analyzed for T CD4+ and CD8+
subsets, separately, by flow cytometry, using a monoclonal antibody
set of 24 different Vβ chains. Compared to eight Brazilian
healthy controls, no differences in Vβ pattern of expression was
observed for patients before or on antiretroviral therapy. Some
chains such as Vβ 3, 14, 16, 20 and 21.3 were over utilized
by both T subsets, independently of HIV infection and/or antiretroviral
treatment, differing from the ones described for individuals
of other nationalities. However, when each patient was
taken individually, particular alterations were detected for the
Vβ gene usage, compared to controls, for all individuals. After
treatment, significantVβ usage changes were observed for seven
patients. One or more chains on both T subsets were engaged
in this process, defining a preferential oligoclonal profile for
TCR repertoire distribution, after HAART. Although no pattern
of specific Vβ changes was detected in the circulating T cells,
we cannot exclude that differential immune responses to HIV or
other important antigens are being focused by these cells.
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