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2022-01-01
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- IOC - Artigos de Periódicos [12500]
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EXPRESSION OF ADHESION MOLECULES IN LUNGS OF MICE INFECTED WITH PARACOCCIDIOIDES BRASILIENSIS CONIDIA
Author
Affilliation
Medical and Experimental Mycology Group. Corporación para Investigaciones Biológicas. Medellin, Colombia / Corporación Ciencias Básicas Biomédicas. Universidad de Antioquia. Medellin, Colombia.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.
Medical and Experimental Mycology Group. Corporación para Investigaciones Biológicas. Medellin, Colombia.
Medical and Experimental Mycology Group. Corporación para Investigaciones Biológicas. Medellin, Colombia.
Medical and Experimental Mycology Group. Corporación para Investigaciones Biológicas. Medellin, Colombia.
Medical and Experimental Mycology Group. Corporación para Investigaciones Biológicas. Medellin, Colombia.
Medical and Experimental Mycology Group. Corporación para Investigaciones Biológicas. Medellin, Colombia / Universidad de Antioquia. Clinical Laboratory and Bcacteriology School. Medellin, Colombia.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.
Medical and Experimental Mycology Group. Corporación para Investigaciones Biológicas. Medellin, Colombia.
Medical and Experimental Mycology Group. Corporación para Investigaciones Biológicas. Medellin, Colombia.
Medical and Experimental Mycology Group. Corporación para Investigaciones Biológicas. Medellin, Colombia.
Medical and Experimental Mycology Group. Corporación para Investigaciones Biológicas. Medellin, Colombia.
Medical and Experimental Mycology Group. Corporación para Investigaciones Biológicas. Medellin, Colombia / Universidad de Antioquia. Clinical Laboratory and Bcacteriology School. Medellin, Colombia.
Abstract
In infected tissues, leukocyte recruitment is mediated by interactions between adhesion molecules, expressed on activated vascular endothelial
cells, and ligands present on circulating cells. We evaluated the inflammatory response and the expression of cellular adhesion molecules
(ICAM-1, VCAM-1, CD18, LFA-1 and Mac-1) in lungs of BALB/c mice infected with Paracoccidioides brasiliensis conidia. When
compared with uninfected animals, infected mice had a significant increase in the inflammatory response during the first 4 days, peaking
2–3 days post-challenge, 40.3% vs. 0.0% and 41.8% vs. 0.7%, respectively. This inflammatory infiltrate was composed mainly of neutrophils
and macrophages with a few eosinophils and lymphocytes. An increase in the intensity of immunofluorescence (IF) for ICAM-1 was also
observed during days 1–4. ICAM-1 was present in bronchiolar epithelium, type II pneumocytes, and macrophages, as well as on vascular
endothelium. The control animals presented ICAM-1 constitutively. In infected mice, VCAM-1 was only observed on vascular endothelium
during the first 2 days, with some macrophages expressing this molecule throughout the study periods. CD18 and Mac-1 but not LFA-1 were
expressed with a high intensity on neutrophils and macrophages present in the inflammatory infiltrate. In addition, we observed a significant
decrease in Colony forming units (CFUs) after the first 2 days post-challenge. These findings suggest that during these early stages, up-regulation
of ICAM-1, VCAM-1, CD18 and Mac-1 expression occurs, participating in the inflammatory process and as such, in the pathogenesis of
paracoccidioidomycosis (PCM).
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