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COMPARISON OF SIX METHODS FOR THE EXTRACTION OF LIPIDS FROM SERUM IN TERMS OF EFFECTIVENESS AND PROTEIN PRESERVATION
Ferraz, T. P. L et al. | Date Issued: 2004
Author
Ferraz, T. P. L
Fiúza, M. C
Santos, M. L. A. dos
Pontes-de-Carvalho, Lain Carlos
Soares, Neci Matos
Affilliation
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Universidade Federal da Bahia. Faculdade de Farmácia. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Universidade Federal da Bahia. Faculdade de Farmácia. Salvador, BA, Brasil.
Abstract
The present work compares six biochemical methods for extraction of lipids from human serum. Although some organic solvents were good lipid extractors, they precipitated most of the total proteins and albumin. On the other hand, methodologies using Triton X-114 and silica were efficient for extraction of lipids, while sparing the protein fraction
Keywords
Lipid extraction
Proteins
Silica
Organic solvents
Detergent
 
DeCS
Técnicas de Laboratório Clínico
Lipídeos
Soro
Polietilenoglicóis
Preservação biológica
Sensibilidade e Especificidade
Proteinas
Dióxido de Silício
Solventes
Humanos
 
Citation
FERRAZ, T.P. et al. Comparison of six methods for the extraction of lipids from serum in terms of effectiveness and protein preservation. Journal of Biochemical and Biophysics Methods, v. 58, n. 3, p. 187-193, mar. 2004.
DOI
doi:10.1016/j.jbbm.2003.10.008
ISSN
0165-022X