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NEUTROPHIL OXIDATIVE METABOLISM AND KILLING OF P. BRASILIENSIS AFTER AIR POUCH INFECTION OF SUSCEPTIBLE AND RESISTANT MICE
Luminescent Measurements
Luminol
Neutrophils
Paracoccidioidomycosis
Paracoccidioides
Medições Luminescentes
Luminol
Neutrófilos
Paracoccidioidomicose
Paracoccidioides
Imunologia
Author
Affilliation
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas e Toxicológicas. São Paulo, SP, Brasil.
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas e Toxicológicas. São Paulo, SP, Brasil.
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas e Toxicológicas. São Paulo, SP, Brasil.
Universidade de São Paulo. Instituto de Ciências Médicas. Departamento de Imunologia. São Paulo, SP, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.
niversidade de São Paulo. Instituto de Ciências Médicas. Departamento de Imunologia. São Paulo, SP, Brasil.
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas e Toxicológicas. São Paulo, SP, Brasil.
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas e Toxicológicas. São Paulo, SP, Brasil.
Universidade de São Paulo. Instituto de Ciências Médicas. Departamento de Imunologia. São Paulo, SP, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.
niversidade de São Paulo. Instituto de Ciências Médicas. Departamento de Imunologia. São Paulo, SP, Brasil.
Abstract
The oxidative burst of polymorphonuclear neutrophils (PMN) and their ability to inhibit Paracoccidioides brasiliensis growth was studied in susceptible (B10.A) and resistant (A/J) mice. The cells were obtained after subcutaneous inoculation in air pouches, yielding highly pure PMN preparations; the number of cells was similar for both strains at 24 h and five times higher in the resistant strain at 15 days. The oxidative metabolism of these PMN was evaluated by the luminol and lucigen-enhanced chemiluminescence upon stimulation with PMA or killed P. brasiliensis (Pb). At 24 h of infection PMN from both strains showed similar responses. However, at 15 days a great enhancement of the Pb-stimulated luminol-enhanced chemiluminescence was observed only in PMN from resistant mice. Such increase was markedly inhibited by the addition of catalase. Independent of the mouse strain or time of infection of lucigen-enhanced chemiluminescence showed the same intensity. The lucigen-enhanced chemiluminescence of PMN without stimuli from resistant mice did not change with the time of infection, however, after 15 days of infection a significantly lower chemiluminescence was detected with PMN from susceptible mice. At 15 days of infection the PMN from B10.A were unable to kill P. brasiliensis yeast cells in vitro. Because the lucigenin- and luminol-enhanced chemiluminescence detects, respectively, the O2- production and the myeloperoxidase/hydrogen peroxide halide system, the present data show parallels between deficiency in the production of oxygen-reactive species by PMN and lower fungicidal activity.
Keywords
Disease SusceptibilityLuminescent Measurements
Luminol
Neutrophils
Paracoccidioidomycosis
Paracoccidioides
DeCS
Suscetibilidade a DoençasMedições Luminescentes
Luminol
Neutrófilos
Paracoccidioidomicose
Paracoccidioides
Imunologia
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