Author | Senna, Simone G. | |
Author | Gomes, Harrison M. | |
Author | Ribeiro, Marta O. | |
Author | Kristki, Afrãnio L. | |
Author | Rossetti, Maria Lucia R. | |
Author | Suffys, Philip N. | |
Access date | 2020-01-30T16:35:57Z | |
Available date | 2020-01-30T16:35:57Z | |
Document date | 2006 | |
Citation | SENNA, Simone G. et al. In house reverse line hybridization assay for rapid detection of susceptibility to rifampicin in isolates of Mycobacterium tuberculosis. Journal of Microbiological Methods, v. 67, p. 385-389, 2006. | pt_BR |
ISSN | 0167-7012 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/39640 | |
Language | por | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | Tuberculose | pt_BR |
Subject in Portuguese | Resistência à rifampicina | pt_BR |
Subject in Portuguese | Ensaio de hibridação reversa | pt_BR |
Subject in Portuguese | Detecção | pt_BR |
Subject in Portuguese | Brasil | pt_BR |
Title | In house reverse line hybridization assay for rapid detection of susceptibility to rifampicin in isolates of Mycobacterium tuberculosis | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.mimet.2006.04.008 | |
Abstract | We developed a Reverse Hybridization Assay (RHA) slightly modified from the Rifoligotyping assay and analyzed the presence
of mutations in a specific region of rpoB gene in 157 isolates (90 rifampin-resistant and 67 rifampin sensitive) of Mycobacterium
tuberculosis from patients attended in South and Southeast region of Brazil. Comparing to standardized drug susceptibility testing
results, the sensitivity and specificity of the RHA was respectively 93% (95% IC: 86.6%–97.2%) and 100% respectively.
Additionally, a high agreement (kappa coefficient 95%) between the RHA assay and sequencing was obtained. Among the 90
rifampicin-resistant isolates, RHA identified point mutations in the following codons: 42 isolates (46.6%) in 531; 29 isolates
(32.2%) in 526, 6 isolates (6.7%) in 516, 3 isolates (3.3%) in 522, 2 isolates (2.2%) in 515, 514, 513 and 1 isolate (1.1%) in 511,
524 and 525. Mutations in different codons were simultaneously identified in 8 isolates (8.9%). The RHA used in the present study
had a high accuracy and can be rapidly performed. However, more reproducible hybridization conditions should be looked for to
increase reliability of mutant probe interpretation. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Centro de Desenvolvimento Cientifico e Tecnológico. Instituto de Pesquisas Biológicas. Porto Alegre, RS, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Instituto de Doenças do Tórax. Programa Acadêmico de Tuberculose Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Centro de Desenvolvimento Cientifico e Tecnológico. Instituto de Pesquisas Biológicas. Porto Alegre, RS, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Tuberculosis | pt_BR |
Subject | Rifampicin resistance | pt_BR |
Subject | Detection | pt_BR |
Subject | Reverse hybridization assay | pt_BR |
Subject | Brazil | pt_BR |
e-ISSN | 1872-8359 | |
Embargo date | 2025-01-01 | |