Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/39934
Title: Warifteine, a bisbenzylisoquinoline alkaloid, decreases immediate allergic and thermal hyperalgesic reactions in sensitized animals
Authors: Costa, H. F.
Santos, C. R. Bezerra
Barbosa Filho, J. M.
Martins, M. A.
Piuvezam, M. R.
Affilliation: Universidade Federal da Paraíba. Departamento de Fisiologia e Patologia. Laboratório de Tecnologia Farmacêutica. João Pessoa, PB, Brasil.
Universidade Federal da Paraíba. Departamento de Fisiologia e Patologia. Laboratório de Tecnologia Farmacêutica. João Pessoa, PB, Brasil.
Universidade Federal da Paraíba. Departamento de Fisiologia e Patologia. Laboratório de Tecnologia Farmacêutica. João Pessoa, PB, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.
Universidade Federal da Paraíba. Departamento de Fisiologia e Patologia. Laboratório de Tecnologia Farmacêutica. João Pessoa, PB, Brasil.
Abstract: Warifteine is a bisbenzylisoquinoline alkaloid isolated from the Cissampelos sympodialis Eichl (Menispermaceae). This plant is used in the folk medicine for the treatment of airway respiratory diseases. A murine model of immediate allergic reaction was used to evaluate warifteine treatment in the IgE production, leukocyte activation, thermal hyperalgesia, mast cell degranulation and scratching behavior. BALB/c mice treated with warifteine (0.4-10 mg/Kg) 1 h before OVA sensitization reduced OVA induced paw edema as well as the OVA-specific IgE serum titers as compared with non-treated and OVA-sensitized animals. Warifteine also reduced the mice death evoked by IgE-dependent anaphylactic shock reaction at 30 min after intravenous OVA challenge. To assess the effect of warifteine treatment on T cell proliferative response, spleen cells from warifteine treated or non-treated and OVA-sensitized animals were evaluated. Spleen cells from warifteine treated animals (2.0 mg/kg) did not proliferate following OVA stimulation as compared with spleen cell cultures from non-treated animals. This response may be related with the increase of NO production as observed in peritoneal macrophage cultures treated with warifteine. Thermal hyperalgesia evoked by IgE or histamine/5-hydroxytryptamine challenge was inhibited on rats at dose of 4.0 mg/kg. Warifteine treatment (0.6 or 6.0 microg/ml) also decreased the IgEalphaDNP-BSA sensitized mast degranulation after DNP-BSA challenge measured by histamine release. In addition, compound 48/80-induced scratching behavior was also sensitive to warifteine treatment. These results demonstrate for the first time that warifteine treatment reduced the allergy-associated responses.
Keywords: Warifteine
Cissampelos sympodialis
Mast cells
Immunoglobulin E
Thermal hyperalgesia
Scratching behavior
keywords: Cissampelos sympodialis
Alcalóide de bisbenzilisoquinolina
Mastócitos
Imunoglobulina E
Comportamento de arranhões
Hiperalgesia térmica
Issue Date: 2008
Publisher: Elsevier
Citation: COSTA, H. F. et al. Warifteine, a bisbenzylisoquinoline alkaloid, decreases immediate allergic and thermal hyperalgesic reactions in sensitized animals. International Immunopharmacology, v. 8, p. 519-525, 2008.
DOI: 10.1016/j.intimp.2007.11.009
ISSN: 1567-5769
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
JBBarbosaFilho_MAMartins_etal_IOC_2008.pdf353.5 kBAdobe PDF    Request a copy



FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.