Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/39936
Title: Leishmania braziliensis: Strain-Specific Modulation of Phagosome Maturation.
Authors: Vieira, Tamara da Silva
Duque, Guillermo Arango
Ory, Kévin
Gontijo, Celia Maria Ferreira
Soares, Rodrigo Pedro Pinto
Descoteaux, Albert
Affilliation: Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil/INRS - Centre Armand-Frappier Santé Biotechnologie. Université du Québec. Laval, QC, Canada
INRS - Centre Armand-Frappier Santé Biotechnologie. Université du Québec. Laval, QC, Canada
INRS - Centre Armand-Frappier Santé Biotechnologie. Université du Québec. Laval, QC, Canada/Université de Rennes 1.CHU Rennes. INSERM. Rennes, France
Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil
INRS - Centre Armand-Frappier Santé Biotechnologie. Université du Québec. Laval, QC, Canada
Abstract: Leishmania (Viannia) braziliensis is responsible for the largest number of American tegumentary leishmaniasis (ATL) in Brazil. ATL can present several clinical forms including typical (TL) and atypical (AL) cutaneous and mucocutaneous (ML) lesions. To identify parasite and host factors potentially associated with these diverse clinical manifestations, we first surveyed the expression of two virulence-associated glycoconjugates, lipophosphoglycan (LPG) and the metalloprotease GP63 by a panel of promastigotes of Leishmania braziliensis (L. braziliensis) strains isolated from patients with different clinical manifestations of ATL and from the sand fly vector. We observed a diversity of expression patterns for both LPG and GP63, which may be related to strain-specific polymorphisms. Interestingly, we noted that GP63 activity varies from strain to strain, including the ability to cleave host cell molecules. We next evaluated the ability of promastigotes from these L. braziliensis strains to modulate phagolysosome biogenesis in bone marrow-derived macrophages (BMM), by assessing phagosomal recruitment of the lysosome-associated membrane protein 1 (LAMP-1) and intraphagosomal acidification. Whereas, three out of six L. braziliensis strains impaired the phagosomal recruitment of LAMP-1, only the ML strain inhibited phagosome acidification to the same extent as the L. donovani strain that was used as a positive control. While decreased phagosomal recruitment of LAMP-1 correlated with higher LPG levels, decreased phagosomal acidification correlated with higher GP63 levels. Finally, we observed that the ability to infect and replicate within host cells did not fully correlate with the inhibition of phagosome maturation. Collectively, our results revealed a diversity of strain-specific phenotypes among L. braziliensis isolates, consistent with the high genetic diversity within Leishmania populations
Keywords: Leishmania braziliensis
virulence
ipophosphoglycan
GP63
macrophage
phagosome
intracellular survival
Issue Date: 2019
Publisher: Frontiers Media SA
Citation: VIEIRA, Tamara da Silva et al. Leishmania braziliensis: Strain-Specific Modulation of Phagosome Maturation. Front Cell Infect Microbiol, v. 9, 319, 2019
DOI: 10.3389/fcimb.2019.00319
ISSN: 2235-2988
Copyright: restricted access
Appears in Collections:MG - IRR - Artigos de Periódicos

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