| Author | Pacheco, Fabiana Santos | |
| Author | Prata, Rhana Berto da Silva | |
| Author | Brandão, Sheila Santos | |
| Author | Ferreira, Helen | |
| Author | Rodrigues, Thais Fernanda | |
| Author | Santos, Jéssica Brandão dos | |
| Author | Silva, Camila Oliveira da | |
| Author | Tavares, Isabella Forasteiro | |
| Author | Mendes, Mayara Abud | |
| Author | Rodrigues, Ana Carolina Duarte Pereira | |
| Author | Machado, Alice de Miranda | |
| Author | Nery, José Augusto da Costa | |
| Author | Amadeu, Thaís Porto | |
| Author | Moraes, Milton Ozório | |
| Author | Sarno, Euzenir Nunes | |
| Author | Schmitz, Veronica | |
| Access date | 2020-03-22T18:57:54Z | |
| Available date | 2020-03-22T18:57:54Z | |
| Document date | 2020 | |
| Citation | PACHECO, Fabiana Santos et al. Erythema Nodosum Leprosum Neutrophil Subset Expressing IL-10R1 Transmigrates into Skin Lesions and Responds to IL-10. ImmunoHorizons, v. 4, p. 47-56, Mar. 2020. | pt_BR |
| ISSN | 2573-7732 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/40455 | |
| Language | eng | pt_BR |
| Publisher | American Associated of Immunologists | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Eritema nodoso hanseniano | pt_BR |
| Subject in Portuguese | Neutrófilos | pt_BR |
| Subject in Portuguese | Transmigração | pt_BR |
| Subject in Portuguese | Lesões cutâneas | pt_BR |
| Subject in Portuguese | IL-10R1 | pt_BR |
| Title | Erythema Nodosum Leprosum Neutrophil Subset Expressing IL-10R1 Transmigrates into Skin Lesions and Responds to IL-10 | pt_BR |
| Type | Article | |
| DOI | 10.4049/immunohorizons.1900088 | |
| Abstract | Erythema nodosum leprosum (ENL) is an inflammatory complication in leprosy. Yet, the involvement of ENL neutrophils in the
inflammatory response against Mycobacterium leprae remains poorly explored. Our primary aim was to investigate the utility of the
surface expression of neutrophil IL-10R1 as an ENL biomarker and, secondarily, to evaluate whether leprosy or healthy M.
leprae–stimulated neutrophils produce cytokines and are able to respond to IL-10. We, in this study, describe a subpopulation of
circulating neutrophils of ENL patients that exclusively expressed IL-10R1, providing evidence that IL-10R1+ neutrophils are present in
ENL lesions. It was also found that ENL neutrophils, but not those of nonreactional leprosy controls, were able to secret detectable
levels of TNF ex vivo and the addition of IL-10 blocked TNF release. It was likewise observed that M. leprae–stimulated, healthy
neutrophils expressed IL-10R1 in vitro, and ENL-linked cytokines were released by M. leprae–cultured neutrophils in vitro. Moreover,
consistent with the presence of a fully functional IL-10R, the addition of IL-10 prevented the release of M. leprae–induced cytokines.
Most importantly, dead M. leprae revealed its superior capacity to induce CCL4 and IL-8 in primary neutrophils over live Mycobacterium,
suggesting that M. leprae may hamper the inflammatory machinery as an immune escape mechanism. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universiade do estado do Rio de Janeiro. Faculdade de Ciências Médicas. Laboratório de Imunopatologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Erythema Nodosum Leprosum | pt_BR |
| Subject | Neutrófilos | pt_BR |
| Subject | IL-10R1 | pt_BR |
| Subject | Skin Lesions | pt_BR |
| Subject | Transmigrate | pt_BR |
