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ERYTHEMA NODOSUM LEPROSUM NEUTROPHIL SUBSET EXPRESSING IL-10R1 TRANSMIGRATES INTO SKIN LESIONS AND RESPONDS TO IL-10
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Abstract
Erythema nodosum leprosum (ENL) is an inflammatory complication in leprosy. Yet, the involvement of ENL neutrophils in the
inflammatory response against Mycobacterium leprae remains poorly explored. Our primary aim was to investigate the utility of the
surface expression of neutrophil IL-10R1 as an ENL biomarker and, secondarily, to evaluate whether leprosy or healthy M.
leprae–stimulated neutrophils produce cytokines and are able to respond to IL-10. We, in this study, describe a subpopulation of
circulating neutrophils of ENL patients that exclusively expressed IL-10R1, providing evidence that IL-10R1+ neutrophils are present in
ENL lesions. It was also found that ENL neutrophils, but not those of nonreactional leprosy controls, were able to secret detectable
levels of TNF ex vivo and the addition of IL-10 blocked TNF release. It was likewise observed that M. leprae–stimulated, healthy
neutrophils expressed IL-10R1 in vitro, and ENL-linked cytokines were released by M. leprae–cultured neutrophils in vitro. Moreover,
consistent with the presence of a fully functional IL-10R, the addition of IL-10 prevented the release of M. leprae–induced cytokines.
Most importantly, dead M. leprae revealed its superior capacity to induce CCL4 and IL-8 in primary neutrophils over live Mycobacterium,
suggesting that M. leprae may hamper the inflammatory machinery as an immune escape mechanism.
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