| Author | Trindade, Gisela Freitas | |
| Author | Lima, Sheila Maria Barbosa de | |
| Author | Britto, Constança | |
| Author | Fernandes-Monteiro, Alice Gomes | |
| Access date | 2020-03-27T03:22:53Z | |
| Available date | 2020-03-27T03:22:53Z | |
| Document date | 2020 | |
| Citation | TRINDADE, Gisele Freitas et al. Detection of Yellow Fever Virus by Quantitative Real-Time PCR (qPCR). In: BIASSONI, Roberto; RASO, Alessandro (org.). Quantitative Real-Time PCR: Methods and Protocols. 2. ed. New York: Human Press, 2020, p. 65-67. | pt_BR |
| ISBN | 978-1-4939-9833-3 | |
| URI | https://www.arca.fiocruz.br/handle/icict/40532 | |
| Sponsorship | This study was funded by grants from FAPERJ (CNE E-26/ 202.931/2015) and PAEF/IOC-Fiocruz/CNPq
(25030.000379/2015-16). C. Britto is a research fellow of CNPq (305589/2015-6). The authors thank the Program for Technological Development in Tools for Health (PDTIS-Fiocruz), Bio-Manguinhos for real-time PCR and DNA sequencing platform facilities, and Heloisa Maria Nogueira Diniz from the Production and Image Processing Service of the Oswaldo Cruz Institute, for preparing the figures. | pt_BR |
| Language | por | pt_BR |
| Publisher | Human Press | pt_BR |
| Rights | restricted access | |
| Title | Detection of Yellow Fever Virus by Quantitative Real-Time PCR (qPCR) | pt_BR |
| Type | Book chapter | |
| DOI | 10.1007/978-1-4939-9833-3_6 | |
| Abstract | The recent resurgence of yellow fever virus (YFV) activity in the tropical regions of Africa and South America has sparked renewed interest in this infamous arboviral disease. Also, the development and production of viral vaccines involve several steps that need the monitoring of viral load throughout the process (antigen production, purification, and inactivation). Currently, these steps are followed by plaque lysis titration assay, whose results take about 7–10 days to come out and thus resulting in a laborious and time-consuming approach. With the advent of quantitative real-time PCR (qPCR), we have a faster method to be applied during vaccine production and also to be effectively used for the diagnosis of YFV infection.
The technique herein standardized proved to be effective for determining YF viral load both in vivo and in vitro, thus becoming a very important tool for laboratory analysis to verify the vaccination status of individuals, beyond acting as a quality control for vaccine production and diagnosis. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Yellow fever virus | pt_BR |
| Subject | qPCR | pt_BR |
| Subject | Viral vaccines | pt_BR |
| Subject | Viral load | pt_BR |
| Subject | Molecular diagnosis | pt_BR |
| Subject | TaqMan fluorogenic probe | pt_BR |
| e-ISSN | 1940-6029 | |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
