Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/40532
Title: Detection of Yellow Fever Virus by Quantitative Real-Time PCR (qPCR)
Authors: Trindade, Gisela Freitas
Lima, Sheila Maria Barbosa de
Britto, Constança
Fernandes-Monteiro, Alice Gomes
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.
Abstract: The recent resurgence of yellow fever virus (YFV) activity in the tropical regions of Africa and South America has sparked renewed interest in this infamous arboviral disease. Also, the development and production of viral vaccines involve several steps that need the monitoring of viral load throughout the process (antigen production, purification, and inactivation). Currently, these steps are followed by plaque lysis titration assay, whose results take about 7–10 days to come out and thus resulting in a laborious and time-consuming approach. With the advent of quantitative real-time PCR (qPCR), we have a faster method to be applied during vaccine production and also to be effectively used for the diagnosis of YFV infection. The technique herein standardized proved to be effective for determining YF viral load both in vivo and in vitro, thus becoming a very important tool for laboratory analysis to verify the vaccination status of individuals, beyond acting as a quality control for vaccine production and diagnosis.
Keywords: Yellow fever virus
qPCR
Viral vaccines
Viral load
Molecular diagnosis
TaqMan fluorogenic probe
Issue Date: 2020
Publisher: Human Press
Citation: TRINDADE, Gisele Freitas et al. Detection of Yellow Fever Virus by Quantitative Real-Time PCR (qPCR). In: BIASSONI, Roberto; RASO, Alessandro (org.). Quantitative Real-Time PCR: Methods and Protocols. 2. ed. New York: Human Press, 2020, p. 65-67.
DOI: 10.1007/978-1-4939-9833-3_6
ISBN: 978-1-4939-9833-3
Copyright: restricted access
Appears in Collections:IOC - Capítulos de Livros

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