Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/4068
Title: Calomys callosus: an alternative model to study fibrosis in Schistosomiasis mansoni: the pathology of the acute phase
Authors: Lenzi, Jane A.
Mota, Ester Maria
Machado, Marcelo Pelajo
Paiva, R. A.
Lenzi, Henrique Leonel
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.
Abstract: Twenty Calomys callosus, Rengger, 1830 (Rodentia-Cricetidae) were studied in the early stage of the acute schistosomal mansoni infection (42nd day). The same number of Swiss Webster mice were used as a comparative standard. Liver and intestinal sections, fixed in formalin-Millonig and embedded in paraffin, were stained with hematoxilin and eosin, PAS-Alcian Blue, pH = 1.0 and 2.5, Lennert's Giemsa, Picrosirius plus polarization microscopy, Periodic acid methanamine silver, Gomori's silver reticulin and resorcin-fuchsin. Immunohistological study (indirect immunofluorescence and peroxidase labeled extravidin-biotin methods) was done with antibodies specific to pro-collagen III, fibronectin, elastin, condroitin-sulfate, tenascin, α smooth muscle actin, vimentin and desmin. The hepatic granulomas were small, reaching only 27 of the volume of the hepatic Swiss Webster granuloma. They were composed mainly by large immature macrophages, often filled by schistosomal pigment, characterizing an exsudative-macrophage granuloma type. The granulomas were situated in the parenchyma and in the portal space. They were often intravascular, poor of extracellular matrix components, except fibronectin and presented, sometimes α smooth muscle actin and vimentin positive cells. The C. callosus intestinal granulomas were similar to Swiss Webster, showing predominance of macrophages. Therefore, the C. callosus acquire very well the Schistosoma mansoni infection, without developing strong hepatic acute granulomatous reaction, suggesting lack of histopathological signs of hypersensitivity.
Keywords: Colomys callosus
Intestine
Granuloma
Extracellular matrix
Fibrosis
Liver
Schistosoma mansoni
DeCS: Schistosoma mansoni
Granuloma
Matriz Extracelular
Fibrose
Issue Date: 1995
Publisher: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
Citation: LENZI, J. A. et al. Calomys callosus: an alternative model to study fibrosis in Schistosomiasis mansoni: the pathology of the acute phase. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v.90, n.2, p.311-318. Apr. 1995.
DOI: http://dx.doi.org/10.1590/S0074-02761995000200034
ISSN: 0074-0276
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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