| Author | Fachado, Alberto | |
| Author | Rodriguez, Alexandro | |
| Author | Angel, Sergio O. | |
| Author | Pinto, Diamar C. | |
| Author | Vila, Iviane | |
| Author | Acosta, Armando | |
| Author | Amendoeira, Regina R. | |
| Author | Lannes-Vieira, Joseli | |
| Access date | 2020-04-11T21:12:00Z | |
| Available date | 2020-04-11T21:12:00Z | |
| Document date | 2003 | |
| Citation | FACHADO, Alberto et al. Protective effect of a naked DNA vaccine cocktail against lethal toxoplasmosis in mice. Vaccine, v. 21, p. 1327-1335, 2003. | pt_BR |
| ISSN | 0264-410X | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/40712 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Toxoplasma gondii | pt_BR |
| Subject in Portuguese | Vacinação de DNA | pt_BR |
| Title | Protective effect of a naked DNA vaccine cocktail against lethal toxoplasmosis in mice | pt_BR |
| Type | Article | |
| DOI | 10.1016/S0264-410X(02)00692-8 | |
| Abstract | BALB/c mice were intramuscularly immunized with low doses (25–50 g) of DNA cocktail containing plasmids encoding the full-length
SAG1/P30 and the 196–561 terminal sequence of ROP2 genes. This immunization resulted in a Th1-type response with predominance of
IgG2a and a specific T-cell proliferation with high levels of interferon-gamma (IFN-) secretion, whereas no IL-4 was detected. Moreover,
DNA cocktail immunization induced a long-lasting protection against a lethal challenge with the highly virulent Toxoplasma gondii RH
strain, whereas low doses of single genes were not protective. These results support further investigations to achieve a multigene anti-T.
gondii DNA vaccine. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de Autoimunidade e Imunorregulação. Rio de Janeiro, RJ, Brasil / “Victoria de Girón” Medicine Institute. Department of Immunology. Laboratory of Molecular Biology. Havana, Cuba. | pt_BR |
| Affilliation | “Victoria de Girón” Medicine Institute. Department of Immunology. Laboratory of Molecular Biology. Havana, Cuba. | pt_BR |
| Affilliation | Carlos G. Malbran Institute. Department of Parasitology. Laboratory of Toxoplasmosis. Buenos Aires, Argentina. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Pharmaceutical and Biologic Laboratory. Tissue Culture Department. Havana, Cuba. | pt_BR |
| Affilliation | Carlos J. Finlay Institute. Department of Molecular Biology. Havana, Cuba. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia. Laboratório de Toxoplasmose. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de Autoimunidade e Imunorregulação. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | DNA vaccination | pt_BR |
| Subject | SAG1/P30/ROP2 | pt_BR |
| Subject | Toxoplasma gondii | pt_BR |
| e-ISSN | 1873-2518 | |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
