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PROTECTIVE EFFECT OF A NAKED DNA VACCINE COCKTAIL AGAINST LETHAL TOXOPLASMOSIS IN MICE
Fachado, Alberto et al. | Date Issued: 2003
Author
Fachado, Alberto
Rodriguez, Alexandro
Angel, Sergio O.
Pinto, Diamar C.
Vila, Iviane
Acosta, Armando
Amendoeira, Regina R.
Lannes-Vieira, Joseli
Affilliation
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de Autoimunidade e Imunorregulação. Rio de Janeiro, RJ, Brasil / “Victoria de Girón” Medicine Institute. Department of Immunology. Laboratory of Molecular Biology. Havana, Cuba.
“Victoria de Girón” Medicine Institute. Department of Immunology. Laboratory of Molecular Biology. Havana, Cuba.
Carlos G. Malbran Institute. Department of Parasitology. Laboratory of Toxoplasmosis. Buenos Aires, Argentina.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
Pharmaceutical and Biologic Laboratory. Tissue Culture Department. Havana, Cuba.
Carlos J. Finlay Institute. Department of Molecular Biology. Havana, Cuba.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia. Laboratório de Toxoplasmose. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de Autoimunidade e Imunorregulação. Rio de Janeiro, RJ, Brasil.
Abstract
BALB/c mice were intramuscularly immunized with low doses (25–50 g) of DNA cocktail containing plasmids encoding the full-length SAG1/P30 and the 196–561 terminal sequence of ROP2 genes. This immunization resulted in a Th1-type response with predominance of IgG2a and a specific T-cell proliferation with high levels of interferon-gamma (IFN-) secretion, whereas no IL-4 was detected. Moreover, DNA cocktail immunization induced a long-lasting protection against a lethal challenge with the highly virulent Toxoplasma gondii RH strain, whereas low doses of single genes were not protective. These results support further investigations to achieve a multigene anti-T. gondii DNA vaccine.
Keywords in Portuguese
Toxoplasma gondii
Vacinação de DNA
 
Keywords
DNA vaccination
SAG1/P30/ROP2
Toxoplasma gondii
 
Publisher
Elsevier
Citation
FACHADO, Alberto et al. Protective effect of a naked DNA vaccine cocktail against lethal toxoplasmosis in mice. Vaccine, v. 21, p. 1327-1335, 2003.
DOI
10.1016/S0264-410X(02)00692-8
ISSN
0264-410X