| Author | Corrêa, Rafael | |
| Author | Silca, Luis Felipe Fonseca | |
| Author | Ribeiro, Dalila Juliana Silva | |
| Author | Almeida, Raquel das Neves | |
| Author | Santos, Igor de Oliveira | |
| Author | Corrêa, Luis Henrique | |
| Author | Sant`Anna, Livia Pimental de | |
| Author | Assunção, Leonardo Santos | |
| Author | Bozza, Patrícia T. | |
| Author | Magalhães, Kelly Grace | |
| Access date | 2020-04-19T19:11:35Z | |
| Available date | 2020-04-19T19:11:35Z | |
| Document date | 2020 | |
| Citation | CORRÊA, Rafael et al. Lysophosphatidylcholine Induces NLRP3 Inflammasome-Mediated Foam Cell Formation and Pyroptosis in Human Monocytes and Endothelial Cells. Frontiers in Immunology, v. 10, Article 2927, p. 1-15, Jan. 2020. | pt_BR |
| ISSN | 1664-3224 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/40879 | |
| Language | eng | pt_BR |
| Publisher | Frontiers Media | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Gotículas lipídicas | pt_BR |
| Subject in Portuguese | Lisofosfatidilcolina | pt_BR |
| Subject in Portuguese | Células de espuma | pt_BR |
| Subject in Portuguese | Inflamamassomas | pt_BR |
| Subject in Portuguese | Monócitos humanos | pt_BR |
| Subject in Portuguese | Células endoteliais | pt_BR |
| Title | Lysophosphatidylcholone induces NLRP3 inflammasome-mediated foam cell formation and pyroptosis in human monocytes and endothelial cells | pt_BR |
| Type | Article | |
| DOI | 10.3389/fimmu.2019.02927 | |
| Abstract | Foam cells are specialized lipid-loaded macrophages derived from monocytes and are
a key pathological feature of atherosclerotic lesions. Lysophosphatidylcholine (LPC)
is a major lipid component of the plasma membrane with a broad spectrum of
proinflammatory activities and plays a key role in atherosclerosis. However, the role of
LPC in lipid droplet (LD) biogenesis and the modulation of inflammasome activation is
still poorly understood. In the present study, we investigated whether LPC can induce
foam cell formation through an analysis of LD biogenesis and determined whether
the cell signaling involved in this process is mediated by the inflammasome activation
pathway in human endothelial cells and monocytes. Our results showed that LPC
induced foam cell formation in both types of cells by increasing LD biogenesis via a
NLRP3 inflammasome-dependent pathway. Furthermore, LPC induced pyroptosis in
both cells and the activation of the inflammasome with IL-1β secretion, which was
dependent on potassium efflux and lysosomal damage in human monocytes. The
present study described the IL-1β secretion and foam cell formation triggered by LPC
via an inflammasome-mediated pathway in human monocytes and endothelial cells.
Our results will help improve our understanding of the relationships among LPC, LD
biogenesis, and NLRP3 inflammasome activation in the pathogenesis of atherosclerosis. | pt_BR |
| Affilliation | Universidade de Brasília. Departamento de Biologia Celular. Laboratório de Imunologia e Inflamação. Brasilia, DF, Brasil. | pt_BR |
| Affilliation | Universidade de Brasília. Departamento de Biologia Celular. Laboratório de Imunologia e Inflamação. Brasilia, DF, Brasil. | pt_BR |
| Affilliation | Universidade de Brasília. Departamento de Biologia Celular. Laboratório de Imunologia e Inflamação. Brasilia, DF, Brasil. | pt_BR |
| Affilliation | Universidade de Brasília. Departamento de Biologia Celular. Laboratório de Imunologia e Inflamação. Brasilia, DF, Brasil. | pt_BR |
| Affilliation | Universidade de Brasília. Departamento de Biologia Celular. Laboratório de Imunologia e Inflamação. Brasilia, DF, Brasil. | pt_BR |
| Affilliation | Universidade de Brasília. Departamento de Biologia Celular. Laboratório de Imunologia e Inflamação. Brasilia, DF, Brasil. | pt_BR |
| Affilliation | Universidade de Brasília. Departamento de Biologia Celular. Laboratório de Imunologia e Inflamação. Brasilia, DF, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade de Brasília. Departamento de Biologia Celular. Laboratório de Imunologia e Inflamação. Brasilia, DF, Brasil. | pt_BR |
| Subject | Lysophosphatidylcholine | pt_BR |
| Subject | Foam cells | pt_BR |
| Subject | Lipid droplet | pt_BR |
| Subject | Inflammasome | pt_BR |
| Subject | NLRP3 | pt_BR |
| Subject | Atherosclerosis | pt_BR |
