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A NEW HIGH-THROUGHPUT TOOL TO SCREEN MOSQUITO-BORNE VIRUSES IN ZIKA VIRUS ENDEMIC/EPIDEMIC AREAS
Moutailler, Sara et al. | Date Issued: 2019
Author
Moutailler, Sara
Yousfi, Lena
Mousson, Laurence
Devillers, Elodie
Vazeille, Marie
Vega-Rúa, Anubis
Perrin, Yvon
Jourdain, Frédéric
Chandre, Fabrice
Cannet, Arnaud
Chantilly, Sandrine
Restrepo, Johana
Guidez, Amandine
Dusfour, Isabelle
Abreu, Filipe Vieira Santos de
Santos, Taissa Pereira dos
Jiolle, Davy
Visser, Tessa M.
Koenraadt, Constantianus J. M.
Wongsokarijo, Merril
Diallo, Mawlouth
Diallo, Diawo
Gaye, Alioune
Boyer, Sébastien
Duong, Veasna
Piorkowski, Géraldine
Paupy, Christophe
Oliveira, Ricardo Lourenço de
Lamballerie, Xavier de
Failloux, Anna-Bella
Affilliation
UMR BIPAR, Animal Health Laboratory, ANSES, INRA, Ecole Nationale Vétérinaire d’Alfort, Université Paris-Est. Paris, France.
UMR BIPAR, Animal Health Laboratory, ANSES, INRA, Ecole Nationale Vétérinaire d’Alfort, Université Paris-Est. Paris, France.
Institut Pasteur. Departement of Virology. Arboviruses and Insect Vectors. Paris, France.
UMR BIPAR, Animal Health Laboratory, ANSES, INRA, Ecole Nationale Vétérinaire d’Alfort, Université Paris-Est. Paris, France.
Institut Pasteur. Departement of Virology. Arboviruses and Insect Vectors. Paris, France.
Institut Pasteur of Guadeloupe–Lieu-dit Morne Jolivière. Laboratory of Vector Control Research. Guadeloupe, France.
CNEV, IRD, 34000. Montpellier, France.
CNEV, IRD, 34000. Montpellier, France / UMR MIVEGEC, IRD-CNRS-Montpellier University. Montpellier, France.
CNEV, IRD, 34000. Montpellier, France / UMR MIVEGEC, IRD-CNRS-Montpellier University. Montpellier, France.
CNEV, IRD, 34000. Montpellier, France.
Collectivité Territoriale de Guyane. Direction de la Démoustication. Cayenne, France.
Collectivité Territoriale de Guyane. Direction de la Démoustication. Cayenne, France.
Institut Pasteur de la Guyane. Unité adaptation et contrôle des vecteurs. Cayenne, France.
Institut Pasteur de la Guyane. Unité adaptation et contrôle des vecteurs. Cayenne, France.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.
UMR MIVEGEC, IRD-CNRS-Montpellier University. Montpellier, France.
UMR MIVEGEC, IRD-CNRS-Montpellier University. Montpellier, France.
Wageningen University & Research. Laboratory of Entomology. Wageningen, The Netherlands.
Wageningen University & Research. Laboratory of Entomology. Wageningen, The Netherlands.
Central Laboratory of the Bureau of Public Health. Paramaribo, Suriname.
Institut Pasteur de Dakar. Unité d’Entomologie Médicale. Dakar, Senegal.
Institut Pasteur de Dakar. Unité d’Entomologie Médicale. Dakar, Senegal.
Institut Pasteur de Dakar. Unité d’Entomologie Médicale. Dakar, Senegal.
Institut Pasteur du Cambodge. Medical Entomology Platform and Virology Unit. Phnom Penh, Cambodia.
Institut Pasteur du Cambodge. Medical Entomology Platform and Virology Unit. Phnom Penh, Cambodia.
Aix Marseille Université. Unité des Virus Emergents. IHUMéditerranée Infection. Marseille, France.
UMR MIVEGEC, IRD-CNRS-Montpellier University. Montpellier, France.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.
Aix Marseille Université. Unité des Virus Emergents. IHUMéditerranée Infection. Marseille, France.
Institut Pasteur. Departement of Virology. Arboviruses and Insect Vectors. Paris, France.
Abstract
Mosquitoes are vectors of arboviruses affecting animal and human health. Arboviruses circulate primarily within an enzootic cycle and recurrent spillovers contribute to the emergence of human-adapted viruses able to initiate an urban cycle involving anthropophilic mosquitoes. The increasing volume of travel and trade offers multiple opportunities for arbovirus introduction in new regions. This scenario has been exemplified recently with the Zika pandemic. To incriminate a mosquito as vector of a pathogen, several criteria are required such as the detection of natural infections in mosquitoes. In this study, we used a high-throughput chip based on the BioMark™ Dynamic arrays system capable of detecting 64 arboviruses in a single experiment. A total of 17,958 mosquitoes collected in Zika-endemic/epidemic countries (Brazil, French Guiana, Guadeloupe, Suriname, Senegal, and Cambodia) were analyzed. Here we show that this new tool can detect endemic and epidemic viruses in different mosquito species in an epidemic context. Thus, this fast and low-cost method can be suggested as a novel epidemiological surveillance tool to identify circulating arboviruses.
Keywords in Portuguese
Vírus trasmitidos por mosquitos
Epidemiologia molecular
Vigilância
Análise microfluida
 
Keywords
Mosquito-borne viruses
Molecular epidemiology
Surveillance
Microfluidic analysis
 
Publisher
MDPI
Citation
MOUTAILLER, Sara et al. A New High-Throughput Tool to Screen Mosquito-Borne Viruses in Zika Virus Endemic/Epidemic Areas. Viruses, v. 11, n. 904, p. 1-19, 2019.
DOI
10.3390/v11100904
ISSN
1999-4915