| Author | Stefani, Mariane Martins de Araújo | |
| Author | Avanzi, Charlotte | |
| Author | Bührer-Sékula, Samira | |
| Author | Benjak, Andrej | |
| Author | Loiseau, Chloé | |
| Author | Singh, Pushpendra | |
| Author | Pontes, Maria Araci de Andrade | |
| Author | Gonçalves, Heitor de Sá | |
| Author | Hungria, Emerith Mayra | |
| Author | Busso, Philippe | |
| Author | Piton, Jérémie | |
| Author | Silveira, Maria I. S. | |
| Author | Cruz, Rossilene | |
| Author | Schetinni, Antônio | |
| Author | Costa, Mauricio Barcelos | |
| Author | Virmond, Marcos da Cunha Lopes | |
| Author | Diorio, Suzana M. | |
| Author | Baptista, Ida Maria Foschiani Dias | |
| Author | Rosa, Patricia Sammarco | |
| Author | Matsuoka, Masanori | |
| Author | Penna, Maria Lucia Fernandes | |
| Author | Cole, Stewart | |
| Author | Penna, Gerson Oliveira | |
| Access date | 2020-05-12T18:46:54Z | |
| Available date | 2020-05-12T18:46:54Z | |
| Document date | 2017 | |
| Citation | STEFANI, Mariane M. A. et al. Whole genome sequencing distinguishes between relapse and reinfection in recurrent leprosy cases. Plos Neglected Tropical Diseases, [San Francisco], v. 11, n. 6, p. 1-13, june 2017. | pt_BR |
| ISSN | 1935-2727 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/41205 | |
| Sponsorship | Fondation Raoul Follerea / The Swiss National Science Foundation / MS-DECIT e Cnpq. | pt_BR |
| Language | eng | pt_BR |
| Publisher | Public Library of Science | pt_BR |
| Rights | open access | pt_BR |
| MeSH | Computational Biology | pt_BR |
| MeSH | DNA, Bacterial | pt_BR |
| MeSH | High-Throughput Nucleotide Sequencing | pt_BR |
| MeSH | Leprosy | pt_BR |
| MeSH | Molecular Typing | pt_BR |
| MeSH | Mycobacterium leprae | pt_BR |
| MeSH | Randomized Controlled Trials as Topic | pt_BR |
| MeSH | Recurrence | pt_BR |
| MeSH | Sequence Analysis, DNA | pt_BR |
| MeSH | Genome, Bacterial | pt_BR |
| Title | Whole genome sequencing distinguishes between relapse and reinfection in recurrent leprosy cases | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1371/journal.pntd.0005598 | |
| Abstract | Background: Since leprosy is both treated and controlled by multidrug therapy (MDT) it is important to monitor recurrent cases for drug resistance and to distinguish between relapse and reinfection as a means of assessing therapeutic efficacy. All three objectives can be reached with single nucleotide resolution using next generation sequencing and bioinformatics analysis of Mycobacterium leprae DNA present in human skin. Methodology: DNA was isolated by means of optimized extraction and enrichment methods from samples from three recurrent cases in leprosy patients participating in an open-label, randomized, controlled clinical trial of uniform MDT in Brazil (U-MDT/CT-BR). Genome-wide sequencing of M. leprae was performed and the resultant sequence assemblies analyzed in silico. Principal findings: In all three cases, no mutations responsible for resistance to rifampicin, dapsone and ofloxacin were found, thus eliminating drug resistance as a possible cause of disease recurrence. However, sequence differences were detected between the strains from the first and second disease episodes in all three patients. In one case, clear evidence was obtained for reinfection with an unrelated strain whereas in the other two cases, relapse appeared more probable. Conclusions/Significance: This is the first report of using M. leprae whole genome sequencing to reveal that treated and cured leprosy patients who remain in endemic areas can be reinfected by another strain. Next generation sequencing can be applied reliably to M. leprae DNA extracted from biopsies to discriminate between cases of relapse and reinfection, thereby providing a powerful tool for evaluating different outcomes of therapeutic regimens and for following disease transmission. | pt_BR |
| Affilliation | Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Goiânia, GO, Brasil. | pt_BR |
| Affilliation | École Polytechnique Fédérale de Lausanne. Global Health Institute. Lausanne, Switzerland. | pt_BR |
| Affilliation | Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Goiânia, GO, Brasil / Universidade Estadual do Amazonas. Manaus, AM, Brasil / Fundação de Medicina Tropical Doutor Heitor Vieira Dourado. Manaus, AM, Brasil. | pt_BR |
| Affilliation | École Polytechnique Fédérale de Lausanne. Global Health Institute. Lausanne, Switzerland. | pt_BR |
| Affilliation | École Polytechnique Fédérale de Lausanne. Global Health Institute. Lausanne, Switzerland / Swiss Tropical and Public Health Institute. Department of Medical Parasitology and Infection Biology. Basel, Switzerland. | pt_BR |
| Affilliation | École Polytechnique Fédérale de Lausanne. Global Health Institute. Lausanne, Switzerland / Maharaja Sayajirao University of Baroda. Department of Microbiology and Biotechnology Centre. Vadodara, India. | pt_BR |
| Affilliation | Centro de Dermatologia Dona Libânia, Fortaleza, CE, Brasil. | pt_BR |
| Affilliation | Centro de Dermatologia Dona Libânia, Fortaleza, CE, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Goiânia, GO, Brasil. | pt_BR |
| Affilliation | École Polytechnique Fédérale de Lausanne. Global Health Institute. Lausanne, Switzerland. | pt_BR |
| Affilliation | École Polytechnique Fédérale de Lausanne. Global Health Institute. Lausanne, Switzerland. | pt_BR |
| Affilliation | Centro de Dermatologia Dona Libânia, Fortaleza, CE, Brasil. | pt_BR |
| Affilliation | Universidade Estadual do Amazonas. Manaus, AM, Brasil / Fundação de Dermatologia Tropical e Venereologia Alfredo da Matta. Manaus, AM, Brasil. | pt_BR |
| Affilliation | Fundação de Dermatologia Tropical e Venereologia Alfredo da Matta. Manaus, AM, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Goiás. Faculdade de Medicina. Goiânia, GO, Brasil. | pt_BR |
| Affilliation | Instituto Lauro de Souza Lima. Bauru, SP, Brasil. | pt_BR |
| Affilliation | Instituto Lauro de Souza Lima. Bauru, SP, Brasil. | pt_BR |
| Affilliation | Instituto Lauro de Souza Lima. Bauru, SP, Brasil. | pt_BR |
| Affilliation | Instituto Lauro de Souza Lima. Bauru, SP, Brasil. | pt_BR |
| Affilliation | National Institute of Infectious Diseases. Leprosy Research Center. Department of Mycobacteriology. Tokyo, Japan / Jyu-kanbo National Museum. Gunma, Japan. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Departamento de Epidemiologia e Bioestatística. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | École Polytechnique Fédérale de Lausanne. Global Health Institute. Lausanne, Switzerland. | pt_BR |
| Affilliation | Universidade de Brasília. Faculdade de Medicina. Núcleo de Medicina Tropical. Brasília, DF, Brasil / Fundação Oswaldo Cruz. Fiocruz Brasília. Brasília, DF, Brasil. | pt_BR |
| Subject | Genome, bacterial | pt_BR |
| Subject | Leprosy | pt_BR |
| Subject | Mycobacterium leprae | pt_BR |
| Subject | Multidrug therapy | pt_BR |
| Subject | Communicable diseases | pt_BR |
| Subject | DNA, bacterial | pt_BR |
| DeCS | Genoma Bacteriano | pt_BR |
| DeCS | Hanseníase | pt_BR |
| DeCS | Mycobacterium leprae | pt_BR |
| DeCS | DNA Bacteriano | pt_BR |
| DeCS | Esquema de Medicação | pt_BR |
| DeCS | Doenças Transmissíveis | pt_BR |
| DeCS | Resultado do Tratamento | pt_BR |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
