Author | Lenzi, Henrique Leonel | |
Author | Mednis, Alma D. | |
Author | Dessein, Alain J. | |
Access date | 2012-06-02T23:40:13Z | |
Available date | 2012-06-02T23:40:13Z | |
Document date | 1985 | |
Citation | LENZI, H. L.; MEDNIS, A.D.; DESSEIN, A. J. Activation of human eosinophils by monokines and lymphokines: source and biochemical characteristics of the eosinophil cytotoxicity-enhancing activity produced by blood mononuclear cells. Cellular Immunology, v.94, n.2, p.333-46, Sep. 1985. | pt_BR |
ISSN | 0008-8749 | |
URI | https://www.arca.fiocruz.br/handle/icict/4127 | |
Language | eng | pt_BR |
Publisher | Elsevier | en |
Rights | restricted access | pt_BR |
Title | Activation of humaneosinophils by monokines and lymphokines: Source and biochemicalcharacteristics of the eosinophilcytotoxicity-enhancingactivityproduced by bloodmononuclearcells | pt_BR |
Type | Article | pt_BR |
DOI | http://dx.doi.org/10.1016/0008-8749(85)90257-6 | |
Abstract | We and others have previously reported that human blood mononuclear cells release in culture certain substances that enhance the capacity of purified human blood eosinophils to kill the antibody-coated larvae of Schistosoma mansoni. The present study shows that this eosinophil cytotoxicity-enhancing activity (ECEA) is released by monocytes and T lymphocytes. Monocytes produce ECEA in resting and in LPS-stimulated cultures; T lymphocytes release such activity when stimulated by mitogens such as concanavalin A. Furthermore, the human monocytic line U-937 also releases ECEA-like activity when stimulated by LPS. The enhancing activity produced by monocytes has been partially characterized: it is sensitive to proteolysis by trypsin, relatively heat stable, and associated with molecules that have an apparent molecular weight of 14,000 to 65,000 daltons and isoelectric points of 3.8-3.9, 4.2, 4.5, 4.8-4.9. This shows that while ECEA produced by monocytes is heterogeneous in size and charge, it is probably different from interleukin 1. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Harvard Medical School.Departments of Pathology and Medicine, Boston, Massachusetts / Brigham and Women's Hospital. Division of Rheumatology and Immunology.Boston, Massachusetts | pt_BR |
Affilliation | INSERM CNRS. Centre d'Immunologiede Marseille-Luminy, Marseille França | pt_BR |
Subject | eosinophil cytotoxicity-enhancing activity | pt_BR |
Subject | Drug effects | pt_BR |
Subject | Eosinophils | pt_BR |
Subject | Monokines | pt_BR |
Subject | Lymphokines | pt_BR |
Subject | Schistosoma mansoni | pt_BR |
Subject | T-Lymphocytes | pt_BR |
Subject | Concanavalin A | pt_BR |
DeCS | Concanavalina A | pt_BR |
DeCS | Citotoxicidade Imunológica | pt_BR |
DeCS | Eosinófilos | pt_BR |
DeCS | Linfocinas | pt_BR |
DeCS | Linfócitos T | pt_BR |
DeCS | Schistosoma mansoni | pt_BR |
DeCS | Monocinas | pt_BR |
DeCS | Linfocinas | pt_BR |