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|Title:||Activation of humaneosinophils by monokines and lymphokines: Source and biochemicalcharacteristics of the eosinophilcytotoxicity-enhancingactivityproduced by bloodmononuclearcells|
|Authors:||Lenzi, Henrique Leonel|
Mednis, Alma D.
Dessein, Alain J.
|Affilliation:||Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.|
Harvard Medical School.Departments of Pathology and Medicine, Boston, Massachusetts / Brigham and Women's Hospital. Division of Rheumatology and Immunology.Boston, Massachusetts
INSERM CNRS. Centre d'Immunologiede Marseille-Luminy, Marseille França
|Abstract:||We and others have previously reported that human blood mononuclear cells release in culture certain substances that enhance the capacity of purified human blood eosinophils to kill the antibody-coated larvae of Schistosoma mansoni. The present study shows that this eosinophil cytotoxicity-enhancing activity (ECEA) is released by monocytes and T lymphocytes. Monocytes produce ECEA in resting and in LPS-stimulated cultures; T lymphocytes release such activity when stimulated by mitogens such as concanavalin A. Furthermore, the human monocytic line U-937 also releases ECEA-like activity when stimulated by LPS. The enhancing activity produced by monocytes has been partially characterized: it is sensitive to proteolysis by trypsin, relatively heat stable, and associated with molecules that have an apparent molecular weight of 14,000 to 65,000 daltons and isoelectric points of 3.8-3.9, 4.2, 4.5, 4.8-4.9. This shows that while ECEA produced by monocytes is heterogeneous in size and charge, it is probably different from interleukin 1.|
|Keywords:||eosinophil cytotoxicity-enhancing activity|
|Citation:||LENZI, H. L.; MEDNIS, A.D.; DESSEIN, A. J. Activation of human eosinophils by monokines and lymphokines: source and biochemical characteristics of the eosinophil cytotoxicity-enhancing activity produced by blood mononuclear cells. Cellular Immunology, v.94, n.2, p.333-46, Sep. 1985.|
|Appears in Collections:||IOC - Artigos de Periódicos|
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