| Author | Antas, Paulo R. Z. | |
| Author | Cardoso, Fernando L. L. | |
| Author | Oliveira, Eliane B. | |
| Author | Gomes, Patrícia K. C. | |
| Author | Cunha, Kátia S. | |
| Author | Sarno, Euzenir N. | |
| Author | Sampaio, Elizabeth P. | |
| Access date | 2020-05-29T13:11:28Z | |
| Available date | 2020-05-29T13:11:28Z | |
| Document date | 2004 | |
| Citation | ANTAS, Paulo R. Z. et al. Whole Blood Assay to Access T Cell-immune responses to Mycobacterium tuberculosis antigens in Health Brazilian individuals. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 99, n. 1, p. 53-55, Feb. 2004. | pt_BR |
| ISSN | 0074-0276 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/41445 | |
| Language | eng | pt_BR |
| Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Tuberculose | pt_BR |
| Subject in Portuguese | PPD | pt_BR |
| Subject in Portuguese | Infecção latente de Tubereculose | pt_BR |
| Subject in Portuguese | Interferon y | pt_BR |
| Subject in Portuguese | Respostas de Células T | pt_BR |
| Title | Whole blood assay to access T Cell-immune responses to Mycobacterium tuberculosis antigens in Healthy Brazilian individuals | pt_BR |
| Type | Article | |
| DOI | 10.1590/S0074-02762004000100009 | pt_BR |
| Abstract | The production of interferon γ (IFNγ) guarantees effective T cell-mediated immunity against Mycobacterium
tuberculosis infection. In the present study, we simply compare the in vitro immune responses to Mycobacterium
antigens in terms of IFNγ production in a total of 10 healthy Brazilian volunteers. Whole blood and mononuclear
cells were cultivated in parallel with PPD, Ag85B, and M. bovis hsp65, and five-days supernatants were harvested
for cytokine detection by ELISA. The inter-assay result was that the overall profile of agreement in response to
antigens was highly correlated (r2 = 0.9266; p = 0.0102). Potential analysis is in current progress to dictate the
usefulness of this method to access the immune responses also in tuberculosis patients and its contacts. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseíase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Hospital Municipal Raphael de Paula e Souza. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Tuberculosis | pt_BR |
| Subject | T cell-responses | pt_BR |
| Subject | Whole blood | pt_BR |
| Subject | PPD | pt_BR |
| Subject | Latent TB infections | pt_BR |
| e-ISSN | 1678-8060 | |
