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WHOLE BLOOD ASSAY TO ACCESS T CELL-IMMUNE RESPONSES TO MYCOBACTERIUM TUBERCULOSIS ANTIGENS IN HEALTHY BRAZILIAN INDIVIDUALS
PPD
Infecção latente de Tubereculose
Interferon y
Respostas de Células T
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Abstract
The production of interferon γ (IFNγ) guarantees effective T cell-mediated immunity against Mycobacterium
tuberculosis infection. In the present study, we simply compare the in vitro immune responses to Mycobacterium
antigens in terms of IFNγ production in a total of 10 healthy Brazilian volunteers. Whole blood and mononuclear
cells were cultivated in parallel with PPD, Ag85B, and M. bovis hsp65, and five-days supernatants were harvested
for cytokine detection by ELISA. The inter-assay result was that the overall profile of agreement in response to
antigens was highly correlated (r2 = 0.9266; p = 0.0102). Potential analysis is in current progress to dictate the
usefulness of this method to access the immune responses also in tuberculosis patients and its contacts.
Keywords in Portuguese
TuberculosePPD
Infecção latente de Tubereculose
Interferon y
Respostas de Células T
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