| Author | Antas, Paulo R. Z. | |
| Author | Oliveira, Eliane B. | |
| Author | Milagres, Alexandre S. | |
| Author | Franken, Kees C. | |
| Author | Ottenhoff, Tom H. M. | |
| Author | Klaster, Paul | |
| Author | Sarno, Euzenir N. | |
| Author | Sampaio, Elizabeth P. | |
| Access date | 2020-05-29T13:37:59Z | |
| Available date | 2020-05-29T13:37:59Z | |
| Document date | 2002 | |
| Citation | ANTAS, Paulo R. Z. et al. Kinetics of T Cell-activation molecules in response to Mycobacterium tuberculosis antigens. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 97, n. 8, p. 1097-1099, Dec. 2002. | pt_BR |
| ISSN | 0074-0276 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/41446 | |
| Language | eng | pt_BR |
| Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz | pt_BR |
| Rights | open access | |
| Subject in Portuguese | CD69 | pt_BR |
| Subject in Portuguese | CD25 | pt_BR |
| Subject in Portuguese | Marcadores de ativação | pt_BR |
| Subject in Portuguese | Citometria de fluxo | pt_BR |
| Title | Kinetics of T Cell-activation Molecules in Response to Mycobacterium tuberculosis Antigens | pt_BR |
| Type | Article | |
| DOI | 10.1590/S0074-02762002000800005 | pt_BR |
| Abstract | The phenotypic features acquired subsequent to antigen-specific stimulation in vitro were evaluated by means of
the kinetic expressions of CD69 and CD25 activation molecules on T lymphocytes and assayed by flow cytometry in
response to PPD, Ag85B, and ferritin in PPD-positive healthy control individuals. In response to PHA, CD69
staining on both CD4+ and CD8+ T cells became initially marked after 4 h, peaked at 24 h, and quickly decreased
after 120 h. For CD25, a latter expression was detected around 8 h, having increased after 96 h. As expected, the
response rate to the mycobacterial antigens was much lower than that to the mitogen. Positive staining was high
after 96 h for CD25 and after 24 h for CD69. CD69 expression was significantly enhanced (p < 0.05) on CD8+ as
compared to CD4+ T cells. High levels were also found between 96-120 h. Regarding Ag85B, CD25+ cells were
mostly CD4+ instead of CD8+ T cells. Moreover, in response to ferritin, a lower CD25 expression was noted. The
present data will allow further characterization of the immune response to new mycobacterial-specific antigens and
their evaluation for possible inclusion in developing new diagnostic techniques for tuberculosis as well in a new
vaccine to prevent the disease. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Hospital Municipal Raphael de Paula e Souza. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Leiden University Medical Center. Department of Immunohematology and Blood Transfusion. Leiden, The Netherlands. | pt_BR |
| Affilliation | Leiden University Medical Center. Department of Immunohematology and Blood Transfusion. Leiden, The Netherlands. | pt_BR |
| Affilliation | Royal Tropical Institute. Department of Biomedical Research. Amsterdan, The Netherlands. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Activation markers | pt_BR |
| Subject | CD69 | pt_BR |
| Subject | CD25 | pt_BR |
| Subject | Flow cytometry | pt_BR |
| e-ISSN | 1678-8060 | |
