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STUDY OF THE STRUCTURAL COMPARISON OF ASPERGILLUS FLAVUS INOCULATED IN PEANUT GRAINS AND INDUCED BY CS-137 GAMMA RADIATION AND ELECTRON BEAM
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Universidade Federal do Rio de Janeiro. Escola de Química. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Escola de Química. Rio de Janeiro, RJ, Brasil.
Universidade Estadual da Zona Oeste. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ, Brasil
Universidade Federal do Rio de Janeiro. Escola de Química. Rio de Janeiro, RJ, Brasil.
Universidade Estadual da Zona Oeste. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ, Brasil
Abstract
This study compared the phenotypes of Aspergillus flavus CMT 00079 inoculated in peanut grains
after induction of Cs 137 gamma radiation and electron beams. Peanut grains were distributed in Petri
dishes and inoculated with the fungal strain and incubated in a BOD germination chamber at 25ºC for
5 days. After 24 hours, the plates were irradiated with Cs137 and absorbed doses of 1.0 KGy with
0.5kGy increase to 8.0 KGy. In electron beam irradiation the absorbed doses were 6.0; 7.0; 7.5 and 8.0
KGy. In both sources the standard 0 KGy plate was maintained. The inactivation doses of Cs 137
strains ranged from 4.5 to 8.0 KGy and with electron beam from 6.0 to 8.0 KGy. Between days 1; 7;
15 after irradiation. Within 15 days after irradiation the subcultures in isolated and irradiated nutrient
medium recovered their fungal growth from 1 to 7.5 KGy with CS 137 and 6.0 to 7.5 KGy with
electron beam. The results showed that the absorbed dose of 8.0 KGy was sufficient to inactivate or
eliminate Aspergillus flavus.
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