| Author | Cruz, Helena Medina | |
| Author | Paula, Vanessa Salete de | |
| Author | Cruz, Juliana Custódio Miguel | |
| Author | Ó, Kycia Maria Rodrigues do | |
| Author | Milagres, Flavio Augusto Pádua | |
| Author | Bastos, Francisco Inácio Pinkusfeld Monteiro | |
| Author | Mota, Jurema Corrêa da | |
| Author | Cruz, Marcelo Santos | |
| Author | Andrade, Tarcisio Matos de | |
| Author | Pollo-Flores, Priscila | |
| Author | Leal, Erotildes | |
| Author | Motta-Castro, Ana Rita Coimbra | |
| Author | Ivantes, Claudia Alexandra Pontes | |
| Author | Bezerra, Cristianne Sousa | |
| Author | Barbosa, Jakeline Ribeiro | |
| Author | Cruz, José Napoleão Monte da | |
| Author | Lewis-Ximenez, Lia Laura | |
| Author | Villar, Livia Melo | |
| Access date | 2020-06-03T20:22:56Z | |
| Available date | 2020-06-03T20:22:56Z | |
| Document date | 2020 | |
| Citation | CRUZ, Helena Medina et al. Evaluation of accuracy of hepatitis B virus antigen and antibody detection and relationship between epidemiological factors using dried blood spot. Journal of Virological Methods, [Amsterdam], v. 277, p. 1-11, Mar. 2020. | en_US |
| ISSN | 0166-0934 | |
| URI | https://www.arca.fiocruz.br/handle/icict/41514 | |
| Language | eng | en_US |
| Publisher | Elsevier | en_US |
| Rights | restricted access | |
| MeSH | Diagnosis | en_US |
| MeSH | Dried blood spot | en_US |
| MeSH | Immunoenzyme Techniques | en_US |
| MeSH | Hepatitis B | en_US |
| Title | Evaluation of accuracy of hepatitis B virus antigen and antibody detection and relationship between epidemiological factors using dried blood spot | en_US |
| Type | Article | |
| DOI | 10.1016/j.jviromet.2019.113798 | |
| Abstract | Dried blood spots (DBS) testing might increase the access for Hepatitis B virus (HBV) diagnosis, but little is known about the performance of these assays in real life conditions. This study aims to evaluate the diagnostic accuracy of HBsAg, anti-HBc and anti-HBs detection in DBS in clinical settings and field studies and to evaluate demographic and risk behaviour according the presence of HBsAg and anti-HBc. Paired sera and DBS samples were obtained from 2309 individuals from 3 groups, defined as follows: G1: clinical setting (n = 5-19), G2: general population (n = 1305) and G3: vulnerable individuals that could be more exposed to blood contact (n = 485). Sera and DBS were tested using commercial enzyme immunoassay (EIA), with some modifications added. Using DBS samples, the specificity values were above 90 % for HBsAg and anti-HBc in all groups and for anti-HBs range from 58.6%-85%. HBsAg testing had the best performance in GI (sensitivity = 84.4 %) and among those samples that the paired serum also presented anti-HBc marker (sensitivity = 91.6 %). High sensitivity of anti-HBc testing in DBS samples was observed in GI (80.8 %) and among HBV active cases (HBsAg+/anti-HBc+) (98.4 %). Testing of anti-HBs in DBS showed the highest sensitivity in GIII (65.5 %), in previous HBV exposed and cured individuals and when serum titers were above 100 IU/mL (86.7 %). DBS samples could be used for screening and prevalence studies for HBsAg and anti-HBc, particularly in clinical settings and among HBV active cases in field studies. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Hospital São Lucas. Petrópolis, RJ, Brasil. | en_US |
| Affilliation | Universidade Federal do Tocantins. Faculdade de Medicina. Palmas, TO, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Comunicação e Informação e Tecnologia Científica em Saúde. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Comunicação e Informação e Tecnologia Científica em Saúde. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Psiquiatria. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil. | en_US |
| Affilliation | Universidade Federal Fluminense. Hospital Universitário Antônio Pedro. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Faculdade de Medicina. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Universidade Federal do Mato Grosso do Sul. Campo Grande, MS, Brasil / Fundação Oswaldo Cruz. Fiocruz Mato Grosso do Sul. Campo Grande, MS, Brasil. | en_US |
| Affilliation | Governo do Municipal de Curitiba. Secretária Municipal da Saúde de Curitiba. Centro de Orientação e Aconselhamento. Curitiba, PR, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil / Instituto Federal de Ciência e Tecnologia do Ceará. Fortaleza, CE, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Fiocruz Brasília. Brasília, DF, Brasil. | en_US |
| Affilliation | Governo do Estado do Ceará. Secretaria Estadual de Saúde. Laboratório Central de Saúde Pública do Ceará. Fortaleza, CE, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil. | en_US |
| Subject | Diagnosis | en_US |
| Subject | Dried blood spot | en_US |
| Subject | Enzyme immunoassay | en_US |
| Subject | Hepatitis B | en_US |
| DeCS | Diagnóstico | en_US |
| DeCS | Teste em Amostras de Sangue Seco | en_US |
| DeCS | Técnicas Imunoenzimáticas | en_US |
| DeCS | Hepatite B | en_US |
| Embargo date | 2022-12-31 | |
