Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/4162
Title: Antibodies to Schistosoma mansoni in human cerebrospinal fluid
Other Titles: Antibodies to Schistosoma mansoni in human cerebrospinal fluid
Authors: Santos, Isis Fernandes Magalhães
Lemaire, Denise Carneiro
Andrade Filho, Antonio de Souza
Queiroz, Aristides Cheto de
Carvalho, Otavio Augusto Moreno
Carmo, Theomira Mauadie de Azevedo
Siqueira, Isadora Cristina de
Andrade, Débora M
Rego, Michely F
Guedes, Ana Paula Torres
Reis, Mitermayer Galvão dos
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Universidade Federal da Bahia. Salvador, BA, Brasil
Universidade Federal da Bahia. Salvador, BA, Brasil
Universidade Federal da Bahia. Salvador, BA, Brasil
Universidade Federal da Bahia. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil
Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil
José Silveira Foundation. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Abstract: Cerebrospinal fluid (CSF) and serum samples from patients suspected of having neuroschistosomiasis (NS) were evaluated by an enzyme-linked immunosorbent assay. Monoclonal antibodies of various immunoglobulin isotypes (IgM, IgA, IgE, total IgG, IgG1, IgG2, IgG3, and IgG4) were used to detect antibodies against Schistosoma mansoni soluble egg antigen (SEA) and soluble worm adult preparation (SWAP). Of the 83 CSF samples tested, 55% were reactive toSEA (26% were reactive only toSEA and 29% tobo th SEA and SWAP), 34% were reactive toSWAP (5% only to SWAP and 29% to both SEA and SWAP), and 40% were not reactive with any antigen. Cases that tested positive for SWAP in CSF and negative in serum were not found. Samples with high specific IgG antibody titers were selected for immunoglobulin isotype profiling. In the CSF samples, the antibodies against SEA and SWAP were mainly IgM, IgG1, and IgG4, although other immunoglobulins were also detected. Interestingly, nine patients had high levels of IgG1 only in the CSF. These results suggest that there is local synthesis of IgG1, and that this isotype could be an important immunologic marker in the diagnosis of NS.
DeCS: Anticorpos Anti-Helmínticos/líquido cefalorraquidiano
Doenças do Sistema Nervoso/líquido cefalorraquidiano
Schistosoma mansoni/imunologia
Esquistossomose/líquido cefalorraquidiano
Adolescente
Adulto
Idoso
Ensaio de Imunoadsorção Enzimática
Isotipos de Imunoglobulinas/imunologia
Doenças do Sistema Nervoso/parasitologia
Esquistossomose/parasitologia
Issue Date: 2003
Citation: MAGALHÃES-SANTOS, I.F. et al. Antibodies to Schistosoma mansoni in human cerebrospinal fluid. American Journal of Tropical Medicine and Hygiene, v. 68, n. 3, p. 294-8, mar. 2003.
Date of defense: 2003
Place of defense: Salvador
Department: Centro de Pesquisas Gonçalo Moniz
Defense institution: Fundação Oswaldo Cruz
ISSN: 0002-9637
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
Magalhaes-Santos IF Antibodies to Schistosoma mansoni ....pdf136.1 kBAdobe PDFThumbnail
View/Open



FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.