Author | Soares, Milena Botelho Pereira | |
Author | Bellintani, Moema Cortizo | |
Author | Ribeiro, Ivone Maria | |
Author | Tomassini, Therezinha Coelho Barbosa | |
Author | Santos, Ricardo Ribeiro dos | |
Access date | 2012-07-18T18:18:01Z | |
Available date | 2012-07-18T18:18:01Z | |
Document date | 2003 | |
Citation | SOARES, M .B. et al. Inhibition of macrophage activation and lipopolysaccaride-induced death by seco-steroids purified from Physalis angulata L. European Journal of Pharmacology, v. 459, n. 1, p. 107-112, jan. 2003 | pt_BR |
ISSN | 0014-2999 | |
xmlui.metadata.dc.identifier.other | S0014-2999(02)02829-7 | |
URI | https://www.arca.fiocruz.br/handle/icict/4190 | |
Language | eng | pt_BR |
Rights | open access | pt_BR |
Title | Inhibition of macrophage activation and lipopolysaccaride-induced death by seco-steroids purified from Physalis angulata L. | pt_BR |
Type | Article | pt_BR |
Defense date | 2003 | |
Departament | Centro de Pesquisas Gonçalo Moniz | pt_BR |
Defense Institution | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz | pt_BR |
Place of Defense | Salvador | pt_BR |
Abstract | Physalis angulata L. is an annual herb widely used in popular medicine for the treatment of a variety of pathologies. Here, we tested
immunomodulatory activities of physalins, seco-steroids purified from P. angulata extracts. Addition of physalins B, F or G, but not D, caused
a reduction in nitric oxide production by macrophages stimulated with lipopolysaccaride and interferon-g. In the presence of physalin B,
macrophages stimulated with lipopolysaccaride, alone or in combination with interferon-g, produced lower levels of tumour necrosis factor
(TNF)-a, interleukin-6 and interleukin-12. The inhibitory activity of physalin B, unlike that of dexamethasone, was not reversed by RU486
[(4-dimethylamino) phenyl-17h-hydroxy-17-(1-propynyl)estra-4,9-dien-3-one], an antiglucocorticoid. Physalin B-treated mice had lower
levels of serum TNF-a than control mice after lipopolysaccaride challenge. More importantly, mice injected with physalins B, F or G survived
after a lethal lipopolysaccaride challenge. These results demonstrate that seco-steroids from P. angulata are potent immunomodulatory
substances and act through a mechanism distinct from that of dexamethasone. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Farmanguinhos. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Subject | Steroid | pt_BR |
Subject | Immunomodulation | pt_BR |
Subject | (Physalis angulata L.) | pt_BR |
Subject | Endotoxic shock | pt_BR |
xmlui.metadata.dc.title.original | Inhibition of macrophage activation and lipopolysaccaride-induced death by seco-steroids purified from Physalis angulata L. | pt_BR |
DeCS | Lipopolissacarídeos/farmacologia | pt_BR |
DeCS | Ativação de Macrófagos/efeitos de drogas | pt_BR |
DeCS | Physalis/química | pt_BR |
DeCS | Fitosteróis/farmacologia | pt_BR |
DeCS | Sobrevivência Celular/efeitos de drogas | pt_BR |
DeCS | Células Cultivadas | pt_BR |
DeCS | Citocinas/sangue | pt_BR |
DeCS | Citocinas/metabolismo | pt_BR |
DeCS | Relação Dose-Resposta a Droga | pt_BR |
DeCS | Antagonistas de Hormônios/farmacologia | pt_BR |
DeCS | Lactonas/farmacologia | pt_BR |
DeCS | Macrófagos Peritoneais/citologia | pt_BR |
DeCS | Camundongos Endogâmicos BALB C | pt_BR |
DeCS | Mifepristona/farmacologia | pt_BR |
DeCS | Oxido Nítrico/sangue | pt_BR |
DeCS | Extratos Vegetais/química | pt_BR |