| Author | Soares, Milena Botelho Pereira | |
| Author | Bellintani, Moema Cortizo | |
| Author | Ribeiro, Ivone Maria | |
| Author | Tomassini, Therezinha Coelho Barbosa | |
| Author | Santos, Ricardo Ribeiro dos | |
| Access date | 2012-07-18T18:18:01Z | |
| Available date | 2012-07-18T18:18:01Z | |
| Document date | 2003 | |
| Citation | SOARES, Milena Botelho Pereira et al. Inhibition of macrophage activation and lipopolysaccaride-induced death by seco-steroids purified from Physalis angulata L. European Journal of Pharmacology, v. 459, n. 1, p. 107-112, jan. 2003. | pt_BR |
| ISSN | 0014-2999 | |
| URI | https://www.arca.fiocruz.br/handle/icict/4190 | |
| Language | eng | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Inhibition of macrophage activation and lipopolysaccaride-induced death by seco-steroids purified from Physalis angulata L. | pt_BR |
| Type | Article | |
| DOI | 10.1016/s0014-2999(02)02829-7 | |
| Abstract | Physalis angulata L. is an annual herb widely used in popular medicine for the treatment of a variety of pathologies. Here, we tested immunomodulatory activities of physalins, seco-steroids purified from P. angulata extracts. Addition of physalins B, F or G, but not D, caused a reduction in nitric oxide production by macrophages stimulated with lipopolysaccaride and interferon-gamma. In the presence of physalin B, macrophages stimulated with lipopolysaccaride, alone or in combination with interferon-gamma, produced lower levels of tumour necrosis factor (TNF)-alpha, interleukin-6 and interleukin-12. The inhibitory activity of physalin B, unlike that of dexamethasone, was not reversed by RU486 [(4-dimethylamino) phenyl-17beta-hydroxy-17-(1-propynyl)estra-4,9-dien-3-one], an antiglucocorticoid. Physalin B-treated mice had lower levels of serum TNF-alpha than control mice after lipopolysaccaride challenge. More importantly, mice injected with physalins B, F or G survived after a lethal lipopolysaccaride challenge. These results demonstrate that seco-steroids from P. angulata are potent immunomodulatory substances and act through a mechanism distinct from that of dexamethasone. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos (Farmanguinhos). Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos (Farmanguinhos). Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil. | |
| Subject | Steroid | pt_BR |
| Subject | Immunomodulation | pt_BR |
| Subject | Endotoxic shock | pt_BR |
| DeCS | Lipopolissacarídeos | pt_BR |
| DeCS | Physalis | pt_BR |
| DeCS | Fitosteróis | pt_BR |
| DeCS | Sobrevivência Celular | pt_BR |
| DeCS | Células Cultivadas | pt_BR |
| DeCS | Citocinas | pt_BR |
| DeCS | Metabolismo | pt_BR |
| DeCS | Relação Dose-Resposta a Droga | pt_BR |
| DeCS | Antagonistas de Hormônios | pt_BR |
| Embargo date | 2030-12-31 | |
