Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/4190
Title: Inhibition of macrophage activation and lipopolysaccaride-induced death by seco-steroids purified from Physalis angulata L.
Other Titles: Inhibition of macrophage activation and lipopolysaccaride-induced death by seco-steroids purified from Physalis angulata L.
Authors: Soares, Milena Botelho Pereira
Bellintani, Moema Cortizo
Ribeiro, Ivone Maria
Tomassini, Therezinha Coelho Barbosa
Santos, Ricardo Ribeiro dos
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Farmanguinhos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Abstract: Physalis angulata L. is an annual herb widely used in popular medicine for the treatment of a variety of pathologies. Here, we tested immunomodulatory activities of physalins, seco-steroids purified from P. angulata extracts. Addition of physalins B, F or G, but not D, caused a reduction in nitric oxide production by macrophages stimulated with lipopolysaccaride and interferon-g. In the presence of physalin B, macrophages stimulated with lipopolysaccaride, alone or in combination with interferon-g, produced lower levels of tumour necrosis factor (TNF)-a, interleukin-6 and interleukin-12. The inhibitory activity of physalin B, unlike that of dexamethasone, was not reversed by RU486 [(4-dimethylamino) phenyl-17h-hydroxy-17-(1-propynyl)estra-4,9-dien-3-one], an antiglucocorticoid. Physalin B-treated mice had lower levels of serum TNF-a than control mice after lipopolysaccaride challenge. More importantly, mice injected with physalins B, F or G survived after a lethal lipopolysaccaride challenge. These results demonstrate that seco-steroids from P. angulata are potent immunomodulatory substances and act through a mechanism distinct from that of dexamethasone.
Keywords: Steroid
Immunomodulation
(Physalis angulata L.)
Endotoxic shock
DeCS: Lipopolissacarídeos/farmacologia
Ativação de Macrófagos/efeitos de drogas
Physalis/química
Fitosteróis/farmacologia
Sobrevivência Celular/efeitos de drogas
Células Cultivadas
Citocinas/sangue
Citocinas/metabolismo
Relação Dose-Resposta a Droga
Antagonistas de Hormônios/farmacologia
Lactonas/farmacologia
Macrófagos Peritoneais/citologia
Camundongos Endogâmicos BALB C
Mifepristona/farmacologia
Oxido Nítrico/sangue
Extratos Vegetais/química
Issue Date: 2003
Citation: SOARES, M .B. et al. Inhibition of macrophage activation and lipopolysaccaride-induced death by seco-steroids purified from Physalis angulata L. European Journal of Pharmacology, v. 459, n. 1, p. 107-112, jan. 2003
Date of defense: 2003
Place of defense: Salvador
Department: Centro de Pesquisas Gonçalo Moniz
Defense institution: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz
ISSN: 0014-2999
Copyright: open access
Appears in Collections:Farmanguinhos - Artigos de Periódicos
BA - IGM - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
Soares MB Inhibition of macrophage activation....pdf190.18 kBAdobe PDFThumbnail
View/Open


FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.