Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/42017
Title: The geraniin-rich extract from Reunion Island endemic medicinal plant phyllanthus phillyreifolius Inhibits Zika and Dengue virus infection at non-toxic effect doses in Zebrafish
Authors: Haddad, Juliano G.
Grauzdytė, Dovilė
Koishi, Andrea Cristine
Viranaicken, Wildriss
Venskutonis, Petras Rimantas
Santos, Claudia Nunes Duarte dos
Desprès, Philippe
Diotel, Nicolas
El Kalamouni, Chaker
Affilliation: Université de la Réunion. Unité Mixte Processus Infectieux en Milieu Insulaire Tropical. Plateforme Technologique CYROI. La Réunion, France. CYROI. La Réunion, France.
Department of Food Science and Technology. Kaunas University of Technology. Kaunas, Lithuania.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.
Université de la Réunion. Unité Mixte Processus Infectieux en Milieu Insulaire Tropical. Plateforme Technologique CYROI. La Réunion, France. CYROI. La Réunion, France.
Department of Food Science and Technology. Kaunas University of Technology. Kaunas, Lithuania.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.
Université de la Réunion. Unité Mixte Processus Infectieux en Milieu Insulaire Tropical. Plateforme Technologique CYROI. La Réunion, France. CYROI. La Réunion, France.
Université de la Réunion. Diabète athérothrombose Thérapies Réunion Océan Indien. La Réunion, France.
Université de la Réunion. Unité Mixte Processus Infectieux en Milieu Insulaire Tropical. Plateforme Technologique CYROI. La Réunion, France. CYROI. La Réunion, France.
Abstract: The mosquito-borne viruses dengue (DENV) and Zika (ZIKV) viruses are two medically important pathogens in tropical and subtropical regions of the world. There is an urgent need of therapeutics against DENV and ZIKV, and medicinal plants are considered as a promising source of antiviral bioactive metabolites. In the present study, we evaluated the ability of Phyllanthus phillyreifolius, an endemic medicinal plant from Reunion Island, to prevent DENV and ZIKV infection in human cells. At non-cytotoxic concentration in vitro, incubation of infected A549 cells with a P. phillyreifolius extract or its major active phytochemical geraniin resulted in a dramatic reduction of virus progeny production for ZIKV as well as four serotypes of DENV. Virological assays showed that P. phillyreifolius extract-mediated virus inhibition relates to a blockade in internalization of virus particles into the host cell. Infectivity studies on ZIKV showed that both P. phillyreifolius and geraniin cause a loss of infectivity of the viral particles. Using a zebrafish model, we demonstrated that administration of P. phillyreifolius and geraniin has no effect on zebrafish locomotor activity while no morbidity nor mortality was observed up to 5 days post-inoculation. Thus, P. phillyreifolius could act as an important source of plant metabolite geraniin which is a promising antiviral compound in the fight against DENV and ZIKV.
Keywords: Zika Virus
Dengue Virus
Plants, Medicinal
Antiviral Agents
Zebrafish
Keywords in spanish: Virus Zika
Virus del Dengue
Plantas Medicinales
Antivirales
Phyllanthus
Pez Cebra
keywords: Phyllanthus phillyreifolius
DeCS: Zika virus
Vírus da Dengue
Plantas Medicinais
Antivirais
Peixe-Zebra
Issue Date: 2020
Publisher: MDPI
Citation: HADDAD, Juliano G. et al. The Geraniin-Rich extract from Reunion Island endemic medicinal plant Phyllanthus phillyreifolius Inhibits Zika and Dengue Virus infection at non-toxic effect doses in Zebrafish. Molecules, v. 25, n. 2316, p. 1-17, 2020.
DOI: 10.3390/molecules25102316
ISSN: 1420-3049
Copyright: open access
Appears in Collections:PR - ICC - Artigos de Periódicos

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