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PIPER ANISUM AS A PROMISING NEW SOURCE OF BIOACTIVE METABOLITES
Agentes antimicrobianos
Antitumoral
Etnofarmacologia ·
Metabolômica
Antimicrobial compounds
Antitumor activity ·
Bioactive metabolites
Ethnopharmacology ·
Metabolomics
Author
Affilliation
Abstract
Piper species are commonly used by indigenous communities to treat several gastrointestinal diseases. In China, they are also
used as an active ingredient in formulae to treat cancer. The objective of the study was to perform a large-scale metabolite
profiling analysis to identify bioactive compounds in Piper anisum. Antioxidant capacity was assessed by the DPPH assay
and total phenolics were assessed by Folin–Ciocalteu’s method. Antimicrobial activity was assessed against several Grampositive
and Gram-negative bacteria, whereas cytotoxicity was assessed against tumor cell lines MCF-7, HCT116, HepG2
and HL-60, and non-tumor cell line MRC-5. The multiplatform metabolite profiling approach encompassed NMR, GC–MS
and LC–MS analyses. P. anisum root extract showed the greatest antioxidant capacity and total phenolic content, followed
by the stem and leaf extracts. P. anisum extracts showed a highly selective antimicrobial profile, being specifically active
against C. albicans (MIC of 500 μg mL−1). Additionally, the root extract (50 μg mL−1) showed the highest cell inhibition
percentages against tumor cell lines MCF-7 (59.5%), HCT116 (49.2%), and HepG2 (61.0%). Forty-eight metabolites were
annotated by GC–MS and 27 by LC–MS. These included alkaloids, carbohydrates, fatty acids, hydrocarbons, organic acids,
phenolic compounds, and terpenes. Taken together, these results showed that P. anisum root extract is a promising source
of bioactive compounds.
Keywords in Portuguese
AntioxidanteAgentes antimicrobianos
Antitumoral
Etnofarmacologia ·
Metabolômica
Keywords
Antioxidant capacityAntimicrobial compounds
Antitumor activity ·
Bioactive metabolites
Ethnopharmacology ·
Metabolomics
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