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2022-01-01
Sustainable Development Goals
03 Saúde e Bem-Estar
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PERSISTENCE OF PARVOVIRUS B19 IN LIVER FROM TRANSPLANTED PATIENTS WITH ACUTE LIVER FAILURE
Alves, Arthur D. R. et al. | Date Issued: 2020
Author
Alves, Arthur D. R.
Melgaço, Juliana G.
Garcia, Rita de Cássia N. C.
Raposo, Jessica V.
Paula, Vanessa S. de
Araújo, Cristina C. V.
Pinto, Marcelo A.
Amado, Luciane A.
Affilliation
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.
Universidade Federal Fluminense. Instituto Biomedico. Departamento de Microbiologia e Parasitologia; Niterói, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil.
Hospital Federal de Bonsucesso. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.
Abstract
Aim: In this study, we investigated the presence of B19V in liver tissues from patients with acute liver failure (ALF) and evaluated the viral activity in infected liver. Methods: Serum and liver samples from 30 patients who underwent liver transplantation for ALF were investigated for B19V infection by real-time PCR, serological tests and examination of B19V mRNA (transcript) expression in the liver. Results: The serum and liver samples from seven patients were B19V DNA positive (103-105 copies/ml). Most of them presented detectable anti-B19V IgG, indicating persistent infection. B19V mRNA was detected in all patients, demonstrating intra-hepatic replication. Conclusion: B19V infection of the liver during the course of non-A-E ALF suggested a role of B19V, which produced the worst outcome in co-infected patients and in patients with cryptogenic ALF, in liver damage.
Keywords in Portuguese
Insuficiência hepática aguda
Diagnóstico
Parvovirus B19
Doença hepática
Reação em Cadeia da Polimerase em Tempo Real
 
Keywords
Acute liver failure
Diagnostic
Parvovirus B19
Liver disease
Real-time PCR
 
Publisher
Future Medicine
Citation
ALVES, Arthur D. R. et al. Persistence of Parvovirus B19 in liver from transplanted patients with acute liver failure. Future Microbiol. v; 15, n.5, p. 307-317, 2020.
DOI
10.2217/fmb-2019-0224
ISSN
1746-0913