| Author | Pauvolid-Corrêa, Alex | |
| Author | Dias, Helver Gonçalves | |
| Author | Maia, Laura Marina Siqueira | |
| Author | Porfírio, Grasiela | |
| Author | Morgado, Thais Oliveira | |
| Author | Santos, Gilberto Sabino | |
| Author | Santa Rita, Paula Helena | |
| Author | Barreto, Wanessa Teixeira Gomes | |
| Author | Macedo, Gabriel Carvalho de | |
| Author | Torres, Jaire Marinho | |
| Author | Nantes, Wesley Arruda Gimenes | |
| Author | Santos, Fillipe Martins | |
| Author | Assis, William Oliveira de | |
| Author | Rucco, Andreza Castro | |
| Author | Yui, Rafael Mamoru dos Santos | |
| Author | Campos, João Bosco Vilela | |
| Author | Silva, Renato Rodrigues Leandro e | |
| Author | Ferreira, Raquel da Silva | |
| Author | Neves, Nilvanei Aparecido da Silva | |
| Author | Costa, Michell Charlles de Souza | |
| Author | Martins, Letícia Ramos | |
| Author | Souza, Emerson Marques de | |
| Author | Carvalho, Michellen dos Santos | |
| Author | Lima, Marina Gonçalves | |
| Author | Alves, Fernanda de Cássia Gonçalves | |
| Author | Riquelme Junior, Luiz Humberto Guimarães | |
| Author | Figueiró, Luan Luiz Batista | |
| Author | Santana, Matheus Fernandes Gomes de S | |
| Author | Santos, Luiz Gustavo Rodrigues Oliveira | |
| Author | Medeiros, Samara Serra | |
| Author | Seino, Larissa Lopes | |
| Author | Miranda, Emily Hime | |
| Author | Linhares, José Henrique Rezende | |
| Author | Santos, Vanessa de Oliveira | |
| Author | Silva, Stephanie Almeida da | |
| Author | Lúcio, Kelly Araujo | |
| Author | Gomes, Viviane Silva | |
| Author | Oliveira, Alexandre de Araújo | |
| Author | Silva, Julia dos Santos | |
| Author | Marques, William de Almeida | |
| Author | Marques, Marcio Schafer | |
| Author | Barros, José Junior França de | |
| Author | Campos, Letícia | |
| Author | Lima, Dinair Couto | |
| Author | Coutinho Netto, Claudia | |
| Author | Strüssmann, Christine | |
| Author | Panella, Nicholas | |
| Author | Hannon, Emily | |
| Author | Macedo, Barbara Cristina de | |
| Author | Almeida, Júlia Ramos | |
| Author | Rineiro, Karen Ramos | |
| Author | Csstro, Maria Carolina Barros de | |
| Author | Campos, Larissa Pratta | |
| Author | Santos, Ana Paula Rosa dos | |
| Author | Souza, Isabelle Marino de | |
| Author | Bianchini, Mateus de Assis | |
| Author | Correa, Sandra Helena Ramiro | |
| Author | Luz, Renato Ordones Baptista | |
| Author | Vieira, Ananda dos Santos | |
| Author | PInto, Luzia Maria de Oliveira | |
| Author | Azeredo, Elzinandes | |
| Author | Figueiredo, Luiz Tadeu Moraes | |
| Author | Alencar, Jeronimo Augusto Fonseca | |
| Author | Lima, Sheila Maria Barbosa de | |
| Author | Herrera, Heitor Miraglia | |
| Author | Shlessarenko, Renata Dezengrini | |
| Author | Santos, Flavia Barreto dos | |
| Author | Fillipis, Ana Maria Bispo de | |
| Author | Salyer, Stephanie | |
| Author | Montgomery, Joel | |
| Author | Komar, Nicholas | |
| Access date | 2020-07-31T18:23:26Z | |
| Available date | 2020-07-31T18:23:26Z | |
| Document date | 2019 | |
| Citation | PAUVOLID-CORRÊA, Alex et al. Zika Virus Surveillance at the Human–Animal Interface in West-Central Brazil, 2017–2018. Viruses, v. 11, n. 1164, p. 1-18, 2019. | pt_BR |
| ISSN | 1999-4915 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/42491 | |
| Description | Alex Pauvolid-Corrêa; Ana Maria Bispo de Filippis. Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Description | Helver Gonçalves Dias; Luzia Maria de Oliveira Pinto; Elzinandes Azeredo; Flavia Barreto dos Santos. Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Description | Emily Hime Miranda; José Henrique Rezende Linhares; Vanessa de Oliveira Santos; Stephanie Almeida da Silva; Kelly Araújo Lúcio; Viviane Silva Gomes; Sheila Maria Barbosa de Lima. Fundação Oswaldo Cruz. Bio-Manguinhos. Laboratório de Tecnologia Virológica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Description | Alexandre de Araújo Oliveira; Julia dos Santos Silva; William de Almeida Marques; Jeronimo Augusto Fonseca Alencar. Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Diptera. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Description | José Junior França de Barros; Letícia Campos. Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Description | Dinair Couto-Lima. Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Description | AFFILIAÇÃO: 1 Laboratório de Flavivírus, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-900, Brazil; abispo@ioc.fiocruz.br 2 Laboratório de Imunologia Viral, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-900, Brazil; helvergd@gmail.com (H.G.D.); mopnogueira@gmail.com (L.M.d.O.P.); naideazeredo@gmail.com (E.A.); flaviab@ioc.fiocruz.br (F.B.d.S.) 3 Laboratório de Virologia, Faculdade de Medicina, Universidade Federal de Mato Grosso (UFMT),
Cuiabá 78060-900, Brazil; lauramsmaia@gmail.com (L.M.S.M.); rah.kell_@hotmail.com (R.d.S.F.); nilvanei@hotmail.com (N.A.d.S.N.); michellcharlles2021@gmail.com (M.C.d.S.C.); le_ramos@hotmail.com (L.R.M.); emersonesem@gmail.com (E.M.d.S.); michellengalex@gmail.com (M.d.S.C.); renatadezengrini@yahoo.com.br (R.D.S.) 4 Laboratório de Biologia Parasitária, Programa de Pós-Graduação em Ciências Ambientais e Sustentabilidade Agropecuária, Universidade Católica Dom Bosco (UCDB), Campo Grande 79117-010, Brazil; grasi_porfirio@hotmail.com (G.P.); carvalhodemacedo@gmail.com (G.C.d.M.); jairemarinho@hotmail.com (J.M.T.); wesley_anantes@hotmail.com (W.A.G.N.); filipemsantos@outlook.com (F.M.S.); william.oliveira.assis@gmail.com (W.O.d.A.); drezacastro.rucco@hotmail.com (A.C.R.); rafayui99@gmail.com (R.M.d.S.Y.); bjoaocampos@gmail.com (J.B.V.C.); renatorleandro@gmail.com (R.R.L.e.S.); herrera@ucdb.br (H.M.H.) 5 Hospital Veterinário, Universidade Federal de Mato Grosso (UFMT), Cuiabá 78060-900, Brazil; thaismorgado@gmail.com (T.O.M.); assisbianchini@gmail.com (M.d.A.B.); correasandrahelena@gmail.com (S.H.R.C.); renato.ordones@outlook.com (R.O.B.L.); ananda.asv@gmail.com (A.d.S.V.)6 Centro de Pesquisa em Virologia, Faculdade de Medicina, Universidade de São Paulo (USP), Ribeirão Preto 14025-099, Brazil; sabinogsj@usp.br (G.S.-S.); marcioschafer@msn.com (M.S.M.); ltmfigue@fmrp.usp.br (L.T.M.F.) 7 Biotério, Universidade Católica Dom Bosco (UCDB), Campo Grande 79117-010, Brazil; paulabiovet@ucdb.br (P.H.S.R.); marina-lima94@outlook.com (M.G.L.); fdecassia.alves@gmail.com (F.d.C.G.A.); bioriquelme@gmail.com (L.H.G.R.-J.); llb.figueiro@gmail.com (L.L.B.F.); matheussantana_21@hotmail.com (M.F.G.d.S.) 8 Laboratório de Ecologia de Populações e do Movimento, Programa de Ecologia e Conservação, Universidade Federal de Mato Grosso do Sul (UFMS), Campo Grande 79070-900, Brazil; wanessatgbarreto@gmail.com (W.T.G.B.); gu_tapirus@hotmail.com (L.G.R.O.S.); samarabio2013@gmail.com (S.S.M.); larissalopesseino@hotmail.com (L.L.S.) 9 Laboratório de Tecnologia Virológica, Bio-Manguinhos, Fiocruz, Rio de Janeiro 21040-900, Brazil; emily@bio.fiocruz.br (E.H.M.); jose.henrique@bio.fiocruz.br (J.H.R.L.);
vanessa.oliveira@bio.fiocruz.br (V.d.O.S.); stephanie.silva@bio.fiocruz.br (S.A.d.S.); kelly.araujo@bio.fiocruz.br (K.A.L.); viviane.gomes@bio.fiocruz.br (V.S.G.); smaria@bio.fiocruz.br (S.M.B.d.L.) 10 Laboratório de Diptera, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-900, Brazil; alexandre.bio.br@gmail.com (A.d.A.O.); juliass@ioc.fiocruz.br (J.d.S.S.); wmarques.bio@gmail.com (W.d.A.M.); jafalencar@gmail.com (J.A.F.A.) 11 Laboratório de Virologia Molecular, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-900, Brazil; barros@ioc.fiocruz.br (J.J.F.d.B.); leticia_bonfim1998@hotmail.com (L.C.) 12 Laboratório de Transmissores de Hematozoários, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-900, Brazil; dcouto@ioc.fiocruz.br 13 Centro de Reabilitação de Animais Silvestres (CRAS), Campo Grande 79037-109, Brazil; ccoutinhonetto@gmail.com 14 Faculdade de Medicina Veterinária, Universidade Federal de Mato Grosso (UFMT), Cuiabá 78060-900, Brazil; christrussmann@gmail.com (C.S.); juh.ramos97@gmail.com (J.R.d.A.); ramosrkaren@gmail.com (K.R.R.); m.carolinac@outlook.com (M.C.B.d.C.); larissapratta@gmail.com (L.P.C.); anapaula.santosrosa@hotmail.com (A.P.R.d.S.); isamarinodesouza@gmail.com (I.M.d.S.) 15 Laboratory of Arbovirus Ecology, Arboviral Diseases Branch, U.S. Centers for Disease Control and Prevention (CDC), Fort Collins, CO 80521, USA; nap4@cdc.gov (N.P.); hannon.emily.r@gmail.com (E.H.) 16 Faculdade de Medicina Veterinária da Universidade de Cuiabá (UNIC), Cuiabá 78065-900, Brazil; barbara_cm02macedo@hotmail.com 17 Global Epidemiology, Laboratory, and Surveillance Branch, Division of Global Health Protection, Center for Global Health, CDC, Atlanta, GA 30333, USA; wig9@cdc.gov (S.S.); ztq9@cdc.gov (J.M.) * Correspondence: pauvolid@gmail.com (A.P.-C.); nck6@cdc.gov (N.K.). | pt_BR |
| Language | eng | pt_BR |
| Publisher | MDPI | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Zika virus | pt_BR |
| Subject in Portuguese | Ciclo enzoótico | pt_BR |
| Subject in Portuguese | Zoonóticos | pt_BR |
| Subject in Portuguese | Teste de neutralização de redução de placas | pt_BR |
| Title | Zika Virus Surveillance at the Human–Animal Interface in West-Central Brazil, 2017–2018 | pt_BR |
| Type | Article | |
| DOI | 10.3390/v11121164 | |
| Abstract | Zika virus (ZIKV) was first discovered in 1947 in Uganda but was not considered a public
health threat until 2007 when it found to be the source of epidemic activity in Asia. Epidemic activity
spread to Brazil in 2014 and continued to spread throughout the tropical and subtropical regions of the
Americas. Despite ZIKV being zoonotic in origin, information about transmission, or even exposure
of non-human vertebrates and mosquitoes to ZIKV in the Americas, is lacking. Accordingly, from
February 2017 to March 2018, we sought evidence of sylvatic ZIKV transmission by sampling whole
blood from approximately 2000 domestic and wild vertebrates of over 100 species in West-Central
Brazil within the active human ZIKV transmission area. In addition, we collected over 24,300
mosquitoes of at least 17 genera and 62 species. We screened whole blood samples and mosquito
pools for ZIKV RNA using pan-flavivirus primers in a real-time reverse-transcription polymerase
chain reaction (RT-PCR) in a SYBR Green platform. Positives were confirmed using ZIKV-specific
envelope gene real-time RT-PCR and nucleotide sequencing. Of the 2068 vertebrates tested, none were
ZIKV positive. Of the 23,315 non-engorged mosquitoes consolidated into 1503 pools tested, 22 (1.5%)
with full data available showed some degree of homology to insect-specific flaviviruses. To identify
previous exposure to ZIKV, 1498 plasma samples representing 62 species of domestic and sylvatic vertebrates were tested for ZIKV-neutralizing antibodies by plaque reduction neutralization test
(PRNT90). From these, 23 (1.5%) of seven species were seropositive for ZIKV and negative for
dengue virus serotype 2, yellow fever virus, and West Nile virus, suggesting potential monotypic
reaction for ZIKV. Results presented here suggest no active transmission of ZIKV in non-human
vertebrate populations or in alternative vector candidates, but suggest that vertebrates around human
populations have indeed been exposed to ZIKV in West-Central Brazil. | pt_BR |
| Affilliation | Múltipla - Ver em Notas. | pt_BR |
| Subject | Zika virus | pt_BR |
| Subject | Enzootic cycle | pt_BR |
| Subject | Zoonotic | pt_BR |
| Subject | Plaque reduction neutralization test (PRNT) | pt_BR |
| e-ISSN | 999-4915 | |
