Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/42491
Title: Zika Virus Surveillance at the Human–Animal Interface in West-Central Brazil, 2017–2018
Authors: Pauvolid-Corrêa, Alex
Dias, Helver Gonçalves
Maia, Laura Marina Siqueira
Porfírio, Grasiela
Morgado, Thais Oliveira
Santos, Gilberto Sabino
Santa Rita, Paula Helena
Barreto, Wanessa Teixeira Gomes
Macedo, Gabriel Carvalho de
Torres, Jaire Marinho
Nantes, Wesley Arruda Gimenes
Santos, Fillipe Martins
Assis, William Oliveira de
Rucco, Andreza Castro
Yui, Rafael Mamoru dos Santos
Campos, João Bosco Vilela
Silva, Renato Rodrigues Leandro e
Ferreira, Raquel da Silva
Neves, Nilvanei Aparecido da Silva
Costa, Michell Charlles de Souza
Martins, Letícia Ramos
Souza, Emerson Marques de
Carvalho, Michellen dos Santos
Lima, Marina Gonçalves
Alves, Fernanda de Cássia Gonçalves
Riquelme Junior, Luiz Humberto Guimarães
Figueiró, Luan Luiz Batista
Santana, Matheus Fernandes Gomes de S
Santos, Luiz Gustavo Rodrigues Oliveira
Medeiros, Samara Serra
Seino, Larissa Lopes
Miranda, Emily Hime
Linhares, José Henrique Rezende
Santos, Vanessa de Oliveira
Silva, Stephanie Almeida da
Lúcio, Kelly Araujo
Gomes, Viviane Silva
Oliveira, Alexandre de Araújo
Silva, Julia dos Santos
Marques, William de Almeida
Marques, Marcio Schafer
Barros, José Junior França de
Campos, Letícia
Lima, Dinair Couto
Coutinho Netto, Claudia
Strüssmann, Christine
Panella, Nicholas
Hannon, Emily
Macedo, Barbara Cristina de
Almeida, Júlia Ramos
Rineiro, Karen Ramos
Csstro, Maria Carolina Barros de
Campos, Larissa Pratta
Santos, Ana Paula Rosa dos
Souza, Isabelle Marino de
Bianchini, Mateus de Assis
Correa, Sandra Helena Ramiro
Luz, Renato Ordones Baptista
Vieira, Ananda dos Santos
PInto, Luzia Maria de Oliveira
Azeredo, Elzinandes
Figueiredo, Luiz Tadeu Moraes
Alencar, Jeronimo Augusto Fonseca
Lima, Sheila Maria Barbosa de
Herrera, Heitor Miraglia
Shlessarenko, Renata Dezengrini
Santos, Flavia Barreto dos
Fillipis, Ana Maria Bispo de
Salyer, Stephanie
Montgomery, Joel
Komar, Nicholas
Affilliation: Ver em notas
Abstract: Zika virus (ZIKV) was first discovered in 1947 in Uganda but was not considered a public health threat until 2007 when it found to be the source of epidemic activity in Asia. Epidemic activity spread to Brazil in 2014 and continued to spread throughout the tropical and subtropical regions of the Americas. Despite ZIKV being zoonotic in origin, information about transmission, or even exposure of non-human vertebrates and mosquitoes to ZIKV in the Americas, is lacking. Accordingly, from February 2017 to March 2018, we sought evidence of sylvatic ZIKV transmission by sampling whole blood from approximately 2000 domestic and wild vertebrates of over 100 species in West-Central Brazil within the active human ZIKV transmission area. In addition, we collected over 24,300 mosquitoes of at least 17 genera and 62 species. We screened whole blood samples and mosquito pools for ZIKV RNA using pan-flavivirus primers in a real-time reverse-transcription polymerase chain reaction (RT-PCR) in a SYBR Green platform. Positives were confirmed using ZIKV-specific envelope gene real-time RT-PCR and nucleotide sequencing. Of the 2068 vertebrates tested, none were ZIKV positive. Of the 23,315 non-engorged mosquitoes consolidated into 1503 pools tested, 22 (1.5%) with full data available showed some degree of homology to insect-specific flaviviruses. To identify previous exposure to ZIKV, 1498 plasma samples representing 62 species of domestic and sylvatic vertebrates were tested for ZIKV-neutralizing antibodies by plaque reduction neutralization test (PRNT90). From these, 23 (1.5%) of seven species were seropositive for ZIKV and negative for dengue virus serotype 2, yellow fever virus, and West Nile virus, suggesting potential monotypic reaction for ZIKV. Results presented here suggest no active transmission of ZIKV in non-human vertebrate populations or in alternative vector candidates, but suggest that vertebrates around human populations have indeed been exposed to ZIKV in West-Central Brazil.
Keywords: Zika virus
Enzootic cycle
Zoonotic
Plaque reduction neutralization test (PRNT)
keywords: Zika virus
Ciclo enzoótico
Zoonóticos
Teste de neutralização de redução de placas
Issue Date: 2019
Publisher: MDPI
Citation: PAUVOLID-CORRÊA, Alex et al. Zika Virus Surveillance at the Human–Animal Interface in West-Central Brazil, 2017–2018. Viruses, v. 11, n. 1164, 18p, 2019.
Description: AFFILIAÇÃO: 1 Laboratório de Flavivírus, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-900, Brazil; abispo@ioc.fiocruz.br 2 Laboratório de Imunologia Viral, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-900, Brazil; helvergd@gmail.com (H.G.D.); lmopnogueira@gmail.com (L.M.d.O.P.); naideazeredo@gmail.com (E.A.); flaviab@ioc.fiocruz.br (F.B.d.S.) 3 Laboratório de Virologia, Faculdade de Medicina, Universidade Federal de Mato Grosso (UFMT), Cuiabá 78060-900, Brazil; lauramsmaia@gmail.com (L.M.S.M.); rah.kell_@hotmail.com (R.d.S.F.); nilvanei@hotmail.com (N.A.d.S.N.); michellcharlles2021@gmail.com (M.C.d.S.C.); le_ramos@hotmail.com (L.R.M.); emersonesem@gmail.com (E.M.d.S.); michellengalex@gmail.com (M.d.S.C.); renatadezengrini@yahoo.com.br (R.D.S.) 4 Laboratório de Biologia Parasitária, Programa de Pós-Graduação em Ciências Ambientais e Sustentabilidade Agropecuária, Universidade Católica Dom Bosco (UCDB), Campo Grande 79117-010, Brazil; grasi_porfirio@hotmail.com (G.P.); carvalhodemacedo@gmail.com (G.C.d.M.); jairemarinho@hotmail.com (J.M.T.); wesley_anantes@hotmail.com (W.A.G.N.); filipemsantos@outlook.com (F.M.S.); william.oliveira.assis@gmail.com (W.O.d.A.); drezacastro.rucco@hotmail.com (A.C.R.); rafayui99@gmail.com (R.M.d.S.Y.); bjoaocampos@gmail.com (J.B.V.C.); renatorleandro@gmail.com (R.R.L.e.S.); herrera@ucdb.br (H.M.H.) 5 Hospital Veterinário, Universidade Federal de Mato Grosso (UFMT), Cuiabá 78060-900, Brazil; thaismorgado@gmail.com (T.O.M.); assisbianchini@gmail.com (M.d.A.B.); correasandrahelena@gmail.com (S.H.R.C.); renato.ordones@outlook.com (R.O.B.L.); ananda.asv@gmail.com (A.d.S.V.)6 Centro de Pesquisa em Virologia, Faculdade de Medicina, Universidade de São Paulo (USP), Ribeirão Preto 14025-099, Brazil; sabinogsj@usp.br (G.S.-S.); marcioschafer@msn.com (M.S.M.); ltmfigue@fmrp.usp.br (L.T.M.F.) 7 Biotério, Universidade Católica Dom Bosco (UCDB), Campo Grande 79117-010, Brazil; paulabiovet@ucdb.br (P.H.S.R.); marina-lima94@outlook.com (M.G.L.); fdecassia.alves@gmail.com (F.d.C.G.A.); bioriquelme@gmail.com (L.H.G.R.-J.); llb.figueiro@gmail.com (L.L.B.F.); matheussantana_21@hotmail.com (M.F.G.d.S.) 8 Laboratório de Ecologia de Populações e do Movimento, Programa de Ecologia e Conservação, Universidade Federal de Mato Grosso do Sul (UFMS), Campo Grande 79070-900, Brazil; wanessatgbarreto@gmail.com (W.T.G.B.); gu_tapirus@hotmail.com (L.G.R.O.S.); samarabio2013@gmail.com (S.S.M.); larissalopesseino@hotmail.com (L.L.S.) 9 Laboratório de Tecnologia Virológica, Bio-Manguinhos, Fiocruz, Rio de Janeiro 21040-900, Brazil; emily@bio.fiocruz.br (E.H.M.); jose.henrique@bio.fiocruz.br (J.H.R.L.); vanessa.oliveira@bio.fiocruz.br (V.d.O.S.); stephanie.silva@bio.fiocruz.br (S.A.d.S.); kelly.araujo@bio.fiocruz.br (K.A.L.); viviane.gomes@bio.fiocruz.br (V.S.G.); smaria@bio.fiocruz.br (S.M.B.d.L.) 10 Laboratório de Diptera, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-900, Brazil; alexandre.bio.br@gmail.com (A.d.A.O.); juliass@ioc.fiocruz.br (J.d.S.S.); wmarques.bio@gmail.com (W.d.A.M.); jafalencar@gmail.com (J.A.F.A.) 11 Laboratório de Virologia Molecular, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-900, Brazil; barros@ioc.fiocruz.br (J.J.F.d.B.); leticia_bonfim1998@hotmail.com (L.C.) 12 Laboratório de Transmissores de Hematozoários, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-900, Brazil; dcouto@ioc.fiocruz.br 13 Centro de Reabilitação de Animais Silvestres (CRAS), Campo Grande 79037-109, Brazil; ccoutinhonetto@gmail.com 14 Faculdade de Medicina Veterinária, Universidade Federal de Mato Grosso (UFMT), Cuiabá 78060-900, Brazil; christrussmann@gmail.com (C.S.); juh.ramos97@gmail.com (J.R.d.A.); ramosrkaren@gmail.com (K.R.R.); m.carolinac@outlook.com (M.C.B.d.C.); larissapratta@gmail.com (L.P.C.); anapaula.santosrosa@hotmail.com (A.P.R.d.S.); isamarinodesouza@gmail.com (I.M.d.S.) 15 Laboratory of Arbovirus Ecology, Arboviral Diseases Branch, U.S. Centers for Disease Control and Prevention (CDC), Fort Collins, CO 80521, USA; nap4@cdc.gov (N.P.); hannon.emily.r@gmail.com (E.H.) 16 Faculdade de Medicina Veterinária da Universidade de Cuiabá (UNIC), Cuiabá 78065-900, Brazil; barbara_cm02macedo@hotmail.com 17 Global Epidemiology, Laboratory, and Surveillance Branch, Division of Global Health Protection, Center for Global Health, CDC, Atlanta, GA 30333, USA; wig9@cdc.gov (S.S.); ztq9@cdc.gov (J.M.) * Correspondence: pauvolid@gmail.com (A.P.-C.); nck6@cdc.gov (N.K.)
DOI: 10.3390/v11121164
ISSN: 1999-4915
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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